Abstract | BACKGROUND: The authors previously observed that antiangiogenic scheduling of photodynamic therapy ( PDT) was effective in causing tumor regression through hemostasis. It would thus be expected that photosensitizer entrapped in polycation liposomes (PCLs) would be efficiently taken up in tumor-derived angiogenic vascular endothelial cells due to the strong electrostatic adhesion between the polycation and the plasma membrane, thus resulting in enhanced phototherapeutic efficacy. METHODS: RESULTS: As a result of PDT on angiogenesis-model mice prepared by the dorsal air sac technique, neovascular destruction after laser irradiation was observed when BPD-MA entrapped in PCLs was used. Furthermore, strong suppression of tumor growth was identified by the PCL-mediated PDT treatment along with a prolonged life span for the mice. Destruction of angiogenic vessels and subsequent tumor cell apoptosis were observed after PCL-mediated PDT treatment in an immunofluorescence study. Interestingly, the biodistribution of the injected BPD-MA that was delivered by PCLs indicated invariable photosensitization levels in tumor tissues. CONCLUSIONS: The study revealed that antiangiogenic PDT treatment using a low dose of BPD-MA entrapped in PCLs efficiently induced the destruction of angiogenic vessels and subsequent tumor suppression by vessel occlusion.
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Authors | Yoshito Takeuchi, Kohta Kurohane, Kanae Ichikawa, Sei Yonezawa, Mamoru Nango, Naoto Oku |
Journal | Cancer
(Cancer)
Vol. 97
Issue 8
Pg. 2027-34
(Apr 15 2003)
ISSN: 0008-543X [Print] United States |
PMID | 12673734
(Publication Type: Journal Article)
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Copyright | Copyright 2003 American Cancer Society. |
Chemical References |
- Amines
- Hydrocarbons
- Liposomes
- Luminescent Proteins
- Photosensitizing Agents
- Platelet Endothelial Cell Adhesion Molecule-1
- Polyamines
- Polyelectrolytes
- Porphyrins
- polycations
- benzoporphyrin D
- Green Fluorescent Proteins
- Polyethyleneimine
- hexadecylamine
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Topics |
- Air Sacs
(blood supply)
- Amines
- Animals
- Apoptosis
- Endothelium, Vascular
- Fluorescent Antibody Technique
- Green Fluorescent Proteins
- Hydrocarbons
- In Situ Nick-End Labeling
- Liposomes
- Luminescent Proteins
(metabolism)
- Male
- Mice
- Mice, Inbred BALB C
- Neovascularization, Pathologic
(drug therapy, metabolism, pathology)
- Photochemotherapy
- Photosensitizing Agents
(therapeutic use)
- Platelet Endothelial Cell Adhesion Molecule-1
(metabolism)
- Polyamines
- Polyelectrolytes
- Polyethyleneimine
(chemistry)
- Porphyrins
(therapeutic use)
- Sarcoma, Experimental
(blood supply, pathology)
- Skin Neoplasms
(blood supply, pathology)
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