Abstract |
A human colon carcinoma cell line KM12-LX, expressing low levels of monoclonal antibody (mAb) FH6 epitope, was transfected with alpha 1,3-fucosyltransferase VI cDNA. Clonal populations with high or intermediate expression levels of the mRNA, shown by RT-PCR (FT6hi and FT6in cells, respectively) were obtained. FT6hi cells were found to express both mAb FH6 and KM93 epitopes by flow-cytometric analysis, whereas FT6in cells expressed mAb FH6 epitopes but not mAb KM93 epitopes. The mAb FH6-binding was abrogated by endo-beta-galactosidase treatment of FT6in, but not FT6hi, cells. FT6hi but not FT6in cells adhered to Chinese-hamster-ovary cells expressing human E-selectin. FT6in cells adhered to sections of mouse liver and the adhesion was blocked by treatment of the cells with endo-beta-galactosidase. The results indicate that endo-beta-galactosidase-sensitive and mAb FH6-reactive carbohydrate chains are generated under the control of expression levels of FUT6 and involved in the adhesion of colon carcinoma cells to liver sections.
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Authors | Akira Kanoh, Masayuki Ota, Hisashi Narimatsu, Tatsuro Irimura |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 303
Issue 3
Pg. 896-901
(Apr 11 2003)
ISSN: 0006-291X [Print] United States |
PMID | 12670495
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- E-Selectin
- Oligosaccharides
- RNA, Messenger
- RNA, Neoplasm
- Sialyl Lewis X Antigen
- Fucosyltransferases
- galactoside 3-fucosyltransferase
- Glycoside Hydrolases
- keratan-sulfate endo-1,4-beta-galactosidase
- beta-Galactosidase
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Topics |
- Animals
- Antibodies, Monoclonal
- CHO Cells
- Cell Adhesion
- Colonic Neoplasms
(enzymology, genetics, immunology, pathology)
- Cricetinae
- E-Selectin
(genetics, metabolism)
- Fucosyltransferases
(genetics)
- Gene Expression
- Glycoside Hydrolases
- Humans
- In Vitro Techniques
- Liver
(metabolism, pathology)
- Mice
- Oligosaccharides
(metabolism)
- RNA, Messenger
(genetics, metabolism)
- RNA, Neoplasm
(genetics, metabolism)
- Sialyl Lewis X Antigen
- Transfection
- Tumor Cells, Cultured
- beta-Galactosidase
(metabolism)
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