Application of a systemic herpes simplex virus type 1 infection in the rat as a tool for sunscreen photoimmunoprotection studies.

Abstract

The application of a novel model for sunscreen photoimmunoprotection studies was assessed using a systemic infection of rats with herpes simplex virus type 1 (HSV-1). Rats were irradiated daily with 1 minimal erythemal/oedematous dose of UVB for 7 consecutive days on their shaved backs with or without application of a broad-spectrum sunscreen (containing TiO2) with a sun protection factor of 10. Subsequently, rats were infected intranasally with HSV. UV exposure prior to HSV infection induced increased severity and incidence of clinical signs of disease, suppression of cellular immune responses as assessed by delayed type hypersensitivity and increased viral load in the brain. The sunscreen provided protection against all these UV-induced effects. We conclude that this novel model is a promising way of testing the immunoprotective qualities of sunscreens, based on the response to a common infectious agent of human subjects.

• Authors: Renate G van der Molen, Johan Garssen, Arja de Klerk, Frans H J Claaus, Mary Norval, Henk van Loveren, Henk K Koerten, A Mieke Mommaas
• Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (Photochem Photobiol Sci) Vol. 1 Issue 8 Pg. 592-6 (Aug 2002) ISSN: 1474-905X [Print] England
• PMID: 12659503 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Sunscreening Agents

Topics

• Animals
• Brain (virology)
• Chlorocebus aethiops
• Herpes Simplex (immunology)
• Herpesvirus 1, Human (isolation & purification)
• Hypersensitivity, Delayed
• Male
• Models, Animal
• Rats
• Rats, Wistar
• Sunburn (immunology, prevention & control)
• Sunscreening Agents
• Vero Cells
• Viral Load