WW domain containing oxidoreductase gene expression is altered in non-small cell lung cancer.

Abstract

WWOX (WW domain containing oxidoreductase), a putative tumor suppressor gene that maps to the common fragile site FRA16D on chromosome 16q23.3-24.1, is altered in breast, esophageal, and ovarian cancer. Because the FRA3B/FHIT locus at 3p14.2 is a preferential target for genetic changes caused by tobacco smoke, we intended to evaluate the status of the FRA16D/WWOX gene in non-small cell lung cancer; we have analyzed 27 paired normal and tumor lung tissues and 8 lung cancer cell lines for WWOX alterations by reverse transcriptase-PCR, loss of heterozygosity, and mutation analysis. Transcripts missing WWOX exons were detected in 7 primary tumors (7 of 27; 25.9%) and 5 of 8 cell lines. In addition, loss of heterozygosity at the WWOX locus was observed in 10 primary tumors (10 of 27; 37.0%). We conclude that WWOX alterations occur in a significant fraction of lung cancers and may contribute to the pathogenesis of non-small cell lung cancer.

• Authors: Sai Yendamuri, Tamotsu Kuroki, Francesco Trapasso, Adam C Henry, Kristoffel R Dumon, Kay Huebner, Noel N Williams, Larry R Kaiser, Carlo M Croce
• Journal: Cancer research (Cancer Res) Vol. 63 Issue 4 Pg. 878-81 (Feb 15 2003) ISSN: 0008-5472 [Print] United States
• PMID: 12591741 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carrier Proteins
• Neoplasm Proteins
• RNA, Messenger
• Oxidoreductases

Topics

• Carcinoma, Non-Small-Cell Lung (enzymology, genetics)
• Carrier Proteins (biosynthesis, genetics)
• Gene Deletion
• Genes, Tumor Suppressor
• Humans
• Loss of Heterozygosity
• Lung Neoplasms (enzymology, genetics, pathology)
• Neoplasm Proteins (biosynthesis, genetics)
• Oxidoreductases (biosynthesis, genetics)
• Point Mutation
• Protein Structure, Tertiary
• RNA, Messenger (biosynthesis, genetics)
• Tumor Cells, Cultured