[Growth inhibition of epidermal growth factor-stimulated human glioblastoma cells by nicardipine in vito].

Abstract

OBJECTIVE:

To study the growth effects of epidermal Growth Factor (EGF) and the calcium channel antagonist nicardipine (NC) on U251 MG, a human malignant glioma cell lime, which has high-affinity EGF receptors.

METHODS:

The growth effects of EGF and NC on U251 MG cultured in serum-free and serum-containing (10% fetal bovine serum, FBS) media respectively were observed by MTT colorimeritric analysis.

RESULTS:

(1) EGF significantly enhanced the growth of U251 MG in a dose-dependent manner in serum-free conditions. The near-maximal effect was obtained at 10 mg.ml-1. The addition of serum obscured this effect. (2) NC decreased U251 MG cells proliferation, especially in serum-containing media, and completely blocked the growth-stimulated effect of EGF. The combinated effects of EGF (10 ng.ml-1) and NC equaled those of NC alone.

CONCLUSIONS:

When serum is absent U251 MG cells showed a pronounced mitogenic response to EGF in a dosedependent manner, which approximated that achieved with 10% FBS. NC suppressed the growth of U251 MG cells and completely blocked the growth-stimulated effects of EGF, it can be used as an adjuvent therapy for human malignant gliomas.

• Authors: C X Huang, B Chen, Y F Zhang
• Journal: Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University (Hunan Yi Ke Da Xue Xue Bao) Vol. 26 Issue 3 Pg. 211-4 (Jun 28 2001) ISSN: 1000-5625 [Print] China
• PMID: 12536683 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents
• Culture Media, Serum-Free
• Epidermal Growth Factor
• Nicardipine

Topics

• Antineoplastic Agents (pharmacology)
• Brain Neoplasms (pathology)
• Cell Division (drug effects)
• Culture Media, Serum-Free
• Epidermal Growth Factor (pharmacology)
• Glioblastoma (pathology)
• Humans
• Nicardipine (pharmacology)
• Tumor Cells, Cultured