A human placental extract: in vivo and in vitro assessments of its melanocyte growth and pigment-inducing activities.

Abstract

BACKGROUND:

The authenticity of various prototype human placental extracts with biological activity, such as that inducing vitiligo repigmentation, is under serious criticism, mainly due to a lack of demonstration at the cellular level. Considering the present worldwide scenario with regard to the occurrence and treatment of vitiligo, a thorough scientific exploration of such extracts should be undertaken.

METHOD:

One such prototype placental preparation was prepared, and was evaluated with regard to its melanogenic action in C57BL/6J mice in vivo and its mitogenic and melanogenic activity on B16F10 mouse melanoma cells and normal human melanocytes in vitro. The extract was applied topically to mice with age-induced prolonged telogenic phase of hair growth (grey body coat hair). Standard 3H-thymidine incorporation and spectrophotometric methods were followed to illustrate mitogenic and melanogenic effects at the cellular level.

RESULTS:

The resurgence of blue skin, followed by shiny black hair, at the regions of application of the extract demonstrated the reversal of the age-induced prolonged telogenic phase of hair growth to the anagenic phase after topical application of the extract on C57BL/6J mice. Further support was obtained from histology where, at the extract-treated sites, the development of new melanogenic centers and hair follicles was observed. During in vitro studies, the vehicle-free extract constituents stimulated both mitogenesis and melanogenesis of B16F10 mouse melanoma cells in a concentration-dependent manner. The cell morphology and extent of melanogenesis also showed significant changes. In addition, two known melanocyte activity-modulating peptides, endothelin-1 (ET-1) and adrenocorticotropic hormone (ACTH), were determined in the extract, chiefly in the total lipid fraction, indicating their effective cutaneous permeation.

CONCLUSIONS:

The extract was found to be a potent mitogen in the in vitro condition and a potent melanogen in both the in vitro and in vivo situations. This strongly suggests its therapeutic potential for the repigmentation of vitiligo patches.

• Authors: Prajnamoy Pal, Shampa Mallick, Samir Kr Mandal, Madhusudan Das, Ajit Kr Dutta, Pijush K Datta, Rabindranath Bera, Ranjan Bhadra
• Journal: International journal of dermatology (Int J Dermatol) Vol. 41 Issue 11 Pg. 760-7 (Nov 2002) ISSN: 0011-9059 [Print] England
• PMID: 12452998 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hepatitis B Surface Antigens
• Melanins
• Placental Extracts

Topics

• Animals
• Enzyme-Linked Immunosorbent Assay
• HIV-1 (isolation & purification)
• Hair (drug effects)
• Hepatitis B Surface Antigens (blood)
• Hepatitis B virus (isolation & purification)
• Melanins (biosynthesis)
• Melanocytes (drug effects)
• Mice
• Mice, Inbred C57BL
• Placenta (virology)
• Placental Extracts (pharmacology)
• Skin Pigmentation (drug effects)