T cell response to 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) in multiple sclerosis patients.

Abstract

T cell responses targeting myelin antigens are possibly involved in the pathogenesis of demyelinating diseases, such as multiple sclerosis (MS). Little is known about human T cell responses to 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), the third most abundant myelin protein. We examined the primary peripheral T cell response to CNPase and characterized CNPase-specific CD4+ long-term T cell lines (TCL) from MS patients and healthy donors. The strongest primary responses were found in two MS patients with very active disease and were directed against CNP(343-373). We identified immunodominant epitope clusters in the regions CNP(343-373) and (356-388) that were recognized in the context of MS-associated HLA-DR2 and DR4 molecules. These data provide the immunological basis for further investigation of CNPase as a potential target self-antigen in MS.

• Authors: P A Muraro, M Kalbus, G Afshar, H F McFarland, R Martin
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 130 Issue 1-2 Pg. 233-42 (Sep 2002) ISSN: 0165-5728 [Print] Netherlands
• PMID: 12225906 (Publication Type: Journal Article)

Chemical References

• Antigens, Surface
• HLA-DR Antigens
• Myelin Proteins
• 2',3'-Cyclic-Nucleotide Phosphodiesterases

Topics

• 2',3'-Cyclic-Nucleotide Phosphodiesterases (immunology)
• Adult
• Antigens, Surface (immunology)
• CD4-Positive T-Lymphocytes (enzymology, immunology)
• Cell Culture Techniques (methods)
• Cell Division (immunology)
• Cells, Cultured
• Female
• HLA-DR Antigens (immunology)
• Humans
• Male
• Middle Aged
• Multiple Sclerosis (enzymology, immunology)
• Myelin Proteins (immunology)
• Phenotype
• T-Lymphocytes (enzymology, immunology)