Epstein-Barr virus-positive Hodgkin/Reed-Sternberg-like B cell in non-hodgkin lymphoma: nucleotide sequence of the amplified immunoglobulin heavy-chain variable region gene by the single-cell polymerase chain reaction technique.

Abstract

We examined nucleotide sequences of Epstein-Barr virus (EBV)-positive Hodgkin/Reed-Sternberg (HRS)-like B cells in a case of diffuse large B-cell lymphoma (DLBCL) and a case of adult T-cell lymphoma (ATL) for single-cell polymerase chain reaction of the immunoglobulin heavy-chain gene variable region (VH gene). HRS-like B cells were scattered in the area irrelevant to the lymphoma infiltrates of DLBCL and in the lymphoma area of ATL. HRS-like B cells were positive for CD20 and CD30 but negative for CD15. EBV presented in HRS-like B cells in both cases but not in any lymphoma cells. VH genes of five HRS-like B cells analyzed in DLBCL were polyclonal and showed in-frame sequences with 0% to 2.8% somatic mutation frequency. In an ATL, VH genes of five HRS-like B cells analyzed were polyclonal and somatically mutated. Four cells carried in-frame rearrangements with 3.5% to 17.7% mutation frequency. One of the VH genes has a one-codon deletion. From the fifth cell, an out-of-frame rearrangement with an insertion and a deletion was obtained. Thus, we showed polyclonal EBV-positive HRS-like B cells in both DLBCL and ATL and that whereas EBV-positive, HRS-like B cells in DLBCL exhibited unmutated and mutated VH gene, those in ATL were found to have a somatically mutated VH gene with/without deletions and/or insertions. The HRS-like B cells may appear because of active EBV infection in a patient who is immunosuppressed from the primary lymphoma.

• Authors: Naoya Nakamura, Naoyoshi Mori, Koichi Ohshima, Tetsuo Kuze, Yuko Hashimoto, Yoshikazu Sasaki, Asumi Shirakawa, Michiko Sato, Masafumi Abe
• Journal: Diagnostic molecular pathology : the American journal of surgical pathology, part B (Diagn Mol Pathol) Vol. 11 Issue 2 Pg. 83-9 (Jun 2002) ISSN: 1052-9551 [Print] United States
• PMID: 12045711 (Publication Type: Journal Article)

Chemical References

• Antigens, CD
• DNA, Neoplasm
• Epstein-Barr virus encoded RNA 1
• Immunoglobulin Heavy Chains
• Immunoglobulin Variable Region
• RNA, Viral

Topics

• Adult
• Antigens, CD (analysis)
• B-Lymphocytes (pathology, virology)
• Base Sequence
• DNA, Neoplasm (analysis)
• Dissection
• Epstein-Barr Virus Infections (pathology)
• Genes, Immunoglobulin
• Herpesvirus 4, Human (genetics, isolation & purification)
• Humans
• Immunoenzyme Techniques
• Immunoglobulin Heavy Chains (genetics)
• Immunoglobulin Variable Region (genetics)
• Immunophenotyping
• In Situ Hybridization
• Lymph Nodes (pathology, virology)
• Lymphoma, Non-Hodgkin (genetics, pathology, virology)
• Lymphoma, T-Cell (genetics, pathology, virology)
• Male
• Micromanipulation
• Molecular Sequence Data
• Polymerase Chain Reaction
• RNA, Viral (analysis)
• Reed-Sternberg Cells (pathology, virology)