Studies on the extra- and intracellular acid-base status and its role on metabolic depression in the land snail Helix lucorum (L.) during estivation.

Abstract

The aim of the present study was to examine the acid-base status of extra- and intracellular fluids and its possible role on the regulation of the metabolic rate of Helix lucorum during prolonged estivation. For this purpose, the rate of oxygen consumption for active and estivating snails was determined. The acid-base status was also examined in the hemolymph and tissues from active and estivating snails acclimated at 25 degrees C. In addition, the buffer values of hemolymph and tissues were determined in order to examine whether there is a change in the snails during estivation. The rate of oxygen consumption decreased significantly within the 1st 10 days of estivation from 122.51+/-10 microl.g(-1).h(-1) to 25.86+/-5.2 microl.g(-1).h(-1), indicating a marked decrease in metabolic rate. P(CO2)increased within the 1st 20 days of estivation from 13.52+/-0.68 mmHg to 25.09+/-2.05 mmHg, while the pH of hemolymph (pH(e)) decreased from 7.72+/-0.04 to 7.44+/-0.06. The level of bicarbonates decreased in the hemolymph of estivating snails, indicating a metabolic acidosis, which was moderate in extracellular fluids. In contrast to pH(e), the intracellular pH (pH(i)) was maintained in the tissues of estivating H. lucorum, indicating a regulation of pH(i) despite the developed hypercapnia. According to the results presented here, it seems that the timing of pH(e) changes does not correlate with the timing of metabolic rate reduction in estivating H. lucorum.

• Authors: B Michaelidis
• Journal: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology (J Comp Physiol B) Vol. 172 Issue 4 Pg. 347-54 (May 2002) ISSN: 0174-1578 [Print] Germany
• PMID: 12037597 (Publication Type: Journal Article)

Chemical References

• Lactic Acid
• Succinic Acid

Topics

• Acid-Base Equilibrium (physiology)
• Animals
• Energy Metabolism (physiology)
• Estivation (physiology)
• Helix, Snails (metabolism)
• Hemolymph (metabolism)
• Hydrogen-Ion Concentration
• Lactic Acid (metabolism)
• Succinic Acid (metabolism)