Abstract |
T-705 (6-fluoro-3-hydroxy-2-pyrazinecarboxamide) has been found to have potent and selective inhibitory activity against influenza virus. In an in vitro plaque reduction assay, T-705 showed potent inhibitory activity against influenza A, B, and C viruses, with 50% inhibitory concentrations (IC(50)s) of 0.013 to 0.48 microg/ml, while it showed no cytotoxicity at concentrations up to 1,000 microg/ml in Madin-Darby canine kidney cells. The selectivity index for influenza virus was more than 2,000. It was also active against a neuraminidase inhibitor-resistant virus and some amantadine-resistant viruses. T-705 showed weak activity against non-influenza virus RNA viruses, with the IC(50)s being higher for non-influenza virus RNA viruses than for influenza virus, and it had no activity against DNA viruses. Orally administered T-705 at 100 mg/kg of body weight/day (four times a day) for 5 days significantly reduced the mean pulmonary virus yields and the rate of mortality in mice infected with influenza virus A/PR/8/34 (3 x 10(2) PFU). These results suggest that T-705 may be a compound that is useful and highly selective against influenza virus infections and that has a mode of action different from those of commercially available drugs, such as amantadine, rimantadine, and neuraminidase inhibitors.
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Authors | Y Furuta, K Takahashi, Y Fukuda, M Kuno, T Kamiyama, K Kozaki, N Nomura, H Egawa, S Minami, Y Watanabe, H Narita, K Shiraki |
Journal | Antimicrobial agents and chemotherapy
(Antimicrob Agents Chemother)
Vol. 46
Issue 4
Pg. 977-81
(Apr 2002)
ISSN: 0066-4804 [Print] United States |
PMID | 11897578
(Publication Type: Journal Article)
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Chemical References |
- Amides
- Antiviral Agents
- Pyrazines
- favipiravir
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Topics |
- Amides
(pharmacology, therapeutic use)
- Animals
- Antiviral Agents
(pharmacology, therapeutic use)
- Cell Line
- Drug Screening Assays, Antitumor
- Humans
- Influenza, Human
(drug therapy, virology)
- Lung
(virology)
- Male
- Mice
- Mice, Inbred BALB C
- Microbial Sensitivity Tests
- Orthomyxoviridae
(drug effects)
- Pyrazines
(pharmacology, therapeutic use)
- Viruses
(drug effects)
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