Suppression of tumor growth by novel peptides homing to tumor-derived new blood vessels.

Abstract

Novel peptides homing to angiogenic vessels were recently isolated from a phage-displayed random pentadecapeptide library. One of the isolated peptides, ASSSYPLIHWRPWAR, significantly suppressed the migration of VEGF-stimulated human umbilical vein endothelial cells. Dendoric ASSSYPLIHWRPWAR-peptide suppressed the formation of new blood vessels in dorsal air sac model mice. Furthermore, ASSSYPLIHWRPWAR-peptide and the fragment peptides containing WRP, which is revealed to be an epitope sequence, significantly suppressed the tumor growth, although 15-mer shuffled peptide derived from ASSSYPLIHWRPWAR and pentapeptides with alanine substitution of each residue of WRP did not. Taken together, ASSSYPLIHWRPWAR-peptide may cause tumor dormancy through inhibition of angiogenesis, and the WRP sequence may be the minimal and essential sequence for this activity.

• Authors: Tomohiro Asai, Mayumi Nagatsuka, Koichi Kuromi, Satoru Yamakawa, Kohta Kurohane, Koichi Ogino, Michinori Tanaka, Takao Taki, Naoto Oku
• Journal: FEBS letters (FEBS Lett) Vol. 510 Issue 3 Pg. 206-10 (Jan 16 2002) ISSN: 0014-5793 [Print] England
• PMID: 11801255 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Angiogenesis Inhibitors
• Endothelial Growth Factors
• Epitopes
• Lymphokines
• Peptide Fragments
• Peptide Library
• Peptides
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors

Topics

• Amino Acid Sequence
• Angiogenesis Inhibitors (administration & dosage, chemistry, pharmacology)
• Animals
• Cell Division (drug effects)
• Cell Movement (drug effects)
• Cells, Cultured
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Drug Screening Assays, Antitumor
• Endothelial Growth Factors (pharmacology)
• Endothelium, Vascular (cytology, drug effects)
• Epitopes
• Humans
• Injections, Subcutaneous
• Lymphokines (pharmacology)
• Melanoma, Experimental (blood supply, drug therapy)
• Mice
• Molecular Sequence Data
• Neoplasm Transplantation
• Neovascularization, Pathologic (drug therapy)
• Peptide Fragments (administration & dosage, chemistry, pharmacology)
• Peptide Library
• Peptides (administration & dosage, chemistry, pharmacology)
• Sarcoma, Experimental (blood supply, drug therapy)
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors