[Fine structure of mummified cells of microorganisms formed under exposure to a chemical analog of anabiosis autoinducer].

Abstract

Under the influence of alkyl hydroxybenzene (C6-AHB) added to cell suspensions at a concentration of (1-5) x 10(-3) M, the cells of Saccharomyces cerevisiae, Micrococcus luteus, and Thioalkalivibrio versutus underwent dramatic changes in the ultrastructural organization of cell membranes, cytoplasm, and inclusions. In yeast suspension, the first changes were observed after 15 min in the structure of slit-like invaginations in the cytoplasmic membrane (CM): they were shortened and thickened. In the subsequent 30 to 60 min, CM ruptures were formed in the regions devoid of intramembrane protein particles and in the slit-like invaginations. After 24 h, complete disintegration of the intracellular membrane structures and conglomeration of the ribosomal part of the cytoplasm occurred. Similar changes were observed on the exposure of gram-positive and gram-negative bacteria to AHB. However, the cell wall in all the microorganisms studied was not destroyed, and in Micrococcus luteus it was even thickened. These mummified forms were preserved as morphologically intact but nonviable cells for more than three years of observations. By their ultrastructural characteristics, these mummified forms of microorganisms were similar to the fossilized microorganisms discovered by us in fibrous kerite. The concept of micromummies was formulated. AHB are supposed to play an important role in the process of fossilization of microorganisms in nature.

• Authors: N E Suzina, A L Muliukin, N G Loĭko, A N Kozlova, V V Dmitriev, A P Shorokhova, V M Gorlenko, V I Duda, G I El'-Registan
• Journal: Mikrobiologiia (Mikrobiologiia) 2001 Nov-Dec Vol. 70 Issue 6 Pg. 776-87 ISSN: 0026-3656 [Print] Russia (Federation)
• Vernacular Title: Tonkaia struktura mumifitsirovannykh kletok mikroorganizmov, obrazuiushchikhsia pod vliianiem khinicheskogo analoga autoinduktora anabioza.
• PMID: 11785134 (Publication Type: Journal Article)

Chemical References

• Phenols
• Phospholipids

Topics

• Adaptation, Physiological
• Ectothiorhodospira (chemistry, ultrastructure)
• Freeze Fracturing
• Frozen Sections
• Intracellular Membranes (ultrastructure)
• Micrococcus luteus (chemistry, ultrastructure)
• Phenols (pharmacology)
• Phospholipids (analysis)
• Saccharomyces cerevisiae (chemistry, ultrastructure)