[Effect of adenovirus type 5 E1A gene on proliferation and metastasis of human lung adenocarcinoma cells].

Abstract

OBJECTIVE:

To investigate the effects of E1A gene on proliferation and metastasis of human lung adenocarcinoma cells.

METHODS:

A mammalian E1A-expressing recombinant constructed in our laboratory, named pcDNA3-E1A, was introduced into human lung adenocarcinoma cell line(Anip 973) by lipofectamine. The cells resistant to G418 were selected. The characteristics of the E1A-expressing Anip 973(Anip 973-E1A) cells were studied in vitro and in vivo, including cell growth rate and doubling-time, colony-forming efficiency on soft agarose, tumorigenicity and metastasis.

RESULTS:

The growth rate of E1A-Anip 973 cells was diminished and their colony-forming efficiency was inhibited significantly. The tumorigenicity in nude mice of the E1A-expressing Anip 973 was markedly suppressed.

CONCLUSION:

E1A suppresses the malignant phenotype of human lung adenocarcinoma cell line. This study provides experimental data for gene therapy of lung cancer with E1A.

• Authors: X Qian, Q Zhao, Z Wang
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 22 Issue 5 Pg. 374-6 (Sep 2000) ISSN: 0253-3766 [Print] China
• PMID: 11778271 (Publication Type: English Abstract, Journal Article)

Chemical References

• Adenovirus E1A Proteins

Topics

• Adenocarcinoma (secondary)
• Adenovirus E1A Proteins (genetics, therapeutic use)
• Animals
• Cell Division (drug effects)
• Disease Models, Animal
• Female
• Genetic Therapy
• Humans
• Immunohistochemistry
• Lung Neoplasms (pathology, prevention & control)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasm Metastasis
• Neoplasm Transplantation
• Neoplasms, Experimental (pathology, prevention & control)
• Transfection
• Xenograft Model Antitumor Assays