Cell cycle arrest and the induction of apoptosis in pancreatic cancer cells exposed to adenosine triphosphate in vitro.

Abstract

Adenosine triphosphate (ATP) has been shown to be an inhibitory or a stimulatory agent for cell growth in various types of cells. Here, we studied the effects of extracellular ATP on two pancreatic cancer cell lines, PK-1 and YAPC established by us. In both cell lines, ATP inhibited cell growth in a time- and dose-dependent manner, whereas the same doses of ATP stimulated DNA synthesis. Flow cytometric analysis of the cells incubated with or without ATP demonstrated the ATP-induced striking increase in cells at S-phase. The same analysis showed also the increase in sub-G0/G1 population in the same analysis and the electrophoretic pattern of DNA showed the occurrence of ATP-induced cell disintegration likely to be apoptosis. We suggest that extracellular ATP is cytotoxic for pancreatic cancer cells because of its induction of cell cycle arrest at S-phase and cell death, possibly apoptosis, overcoming the promotion of the entry into S-phase.

• Authors: Takayuki Yamada, Fumikazu Okajima, Mohammed Akbar, Hideaki Tomura, Torao Narita, Tatsuya Yamada, Susumu Ohwada, Yasuo Morishita, Yoichi Kondo
• Journal: Oncology reports (Oncol Rep) 2002 Jan-Feb Vol. 9 Issue 1 Pg. 113-7 ISSN: 1021-335X [Print] Greece
• PMID: 11748467 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• Formazans
• Tetrazolium Salts
• MTT formazan
• Adenosine Triphosphate
• Thymidine

Topics

• Adenosine Triphosphate (pharmacology)
• Apoptosis (drug effects)
• Cell Cycle (drug effects)
• Cell Division (drug effects)
• DNA, Neoplasm (drug effects)
• Flow Cytometry
• Formazans
• Humans
• Pancreatic Neoplasms (drug therapy, pathology)
• Tetrazolium Salts
• Thymidine (metabolism)
• Tumor Cells, Cultured (drug effects)