Expression of angiogenesis-related genes in ovarian carcinoma--a clinicopathologic study.

Abstract

Angiogenic factors play a role in tumor growth and spread. The object of this study was to analyze the correlation between mRNA expression of angiogenesis-related genes and disease outcome in advanced-stage ovarian carcinomas. Sections from 66 primary ovarian carcinomas and metastatic lesions from 41 patients diagnosed with advanced stage ovarian carcinoma (FIGO stages III-IV) were evaluated for expression of basic fibroblast factor (bFGF), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) using mRNA In Situ Hybridization (ISH). Patients were divided in two groups based on disease outcome. Long-term survivors (17 patients) and short-term survivors (24 patients) were defined using a double cut-off of 36 months for disease-free survival (DFS) and 60 months for overall survival (OS). Mean follow-up period was 70 months. The mean values for DFS and OS were 116 and 133 months for long-term survivors, as compared to 3 and 21 months for short-term survivors, respectively. Expression of bFGF mRNA, most often intense, was detected in tumor and stromal cells in the majority of cases. Weak expression of IL-8 mRNA was detected in both cell compartments, while VEGF mRNA expression was limited to few cases. Primary tumors displayed higher bFGF and IL-8 mRNA expression. However, these differences did not reach statistical significance (P > 0.05). bFGF, IL-8 and VEGF mRNA expression in both tumor and stromal cells was comparable in tumors of long-term and short-term survivors, and showed no correlation with disease outcome in survival analysis (P > 0.05). bFGF is the major angiogenic factor expressed in ovarian carcinoma at the mRNA level. mRNA expression of VEGF, bFGF, and IL-8 does not appear to be a predictor of disease outcome in advanced-stage ovarian carcinoma.

• Authors: B Davidson, I Goldberg, J Kopolovic, W H Gotlieb, V Givant-Horwitz, J M Nesland, A Berner, G Ben-Baruch, M Bryne, R Reich
• Journal: Clinical & experimental metastasis (Clin Exp Metastasis) Vol. 18 Issue 6 Pg. 501-7 ( 2000) ISSN: 0262-0898 [Print] Netherlands
• PMID: 11592307 (Publication Type: Journal Article)

Chemical References

• DNA Primers
• Endothelial Growth Factors
• Interleukin-8
• Lymphokines
• RNA, Messenger
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors
• Fibroblast Growth Factor 2

Topics

• Adult
• Aged
• Aged, 80 and over
• Base Sequence
• DNA Primers
• Disease-Free Survival
• Endothelial Growth Factors (genetics)
• Female
• Fibroblast Growth Factor 2 (genetics)
• Humans
• In Situ Hybridization
• Interleukin-8 (genetics)
• Lymphokines (genetics)
• Middle Aged
• Neovascularization, Pathologic (genetics)
• Ovarian Neoplasms (blood supply, genetics, pathology)
• RNA, Messenger (genetics)
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors