Heat shock protein 70 expression increased in rabbit müller cells in the ischemia-reperfusion model.

Abstract

Heat shock protein 70 (HSP70) expression in the rabbit retina was evaluated in the ischemia-reperfusion model. Ischemia was induced by increasing the pressure in the anterior chamber, and the retinas were collected 1 or 12 h after reperfusion. HSP70 expression was examined by Western blot, confocal laser scanning microscopy and immunoelectron microscopy. Western blot showed that HSP70 was significantly increased in the retina 12 h after reperfusion, compared with normal control or 1 h after reperfusion. Confocal microscopy demonstrated that HSP70 was constitutive in Müller cells and that its expression was increased 12 h after reperfusion. Electron microscopy showed that glycogen-like granules were significantly decreased with prominent endoplasmic reticulum 1 h after reperfusion. Twelve hours after reperfusion, however, the density of glycogen-like granules was restored, and immunoelectron microscopy indicated that HSP70 was associated with the organelles of the Müller cells. Taken together, these data suggest that HSP70 in Müller cells plays a role in accommodation to stress.

• Authors: T Gohdo, H Ueda, S Ohno, H Iijima, S Tsukahara
• Journal: Ophthalmic research (Ophthalmic Res) 2001 Sep-Oct Vol. 33 Issue 5 Pg. 298-302 ISSN: 0030-3747 [Print] Switzerland
• PMID: 11586064 (Publication Type: Journal Article)
• Copyright: Copyright 2001 S. Karger AG, Basel

Chemical References

• HSP70 Heat-Shock Proteins

Topics

• Animals
• Blotting, Western
• HSP70 Heat-Shock Proteins (metabolism)
• Microscopy, Confocal
• Microscopy, Immunoelectron
• Neuroglia (metabolism, ultrastructure)
• Rabbits
• Reperfusion Injury (metabolism, pathology)
• Retina (metabolism, ultrastructure)
• Retinal Diseases (metabolism, pathology)