Role of linkage specific 9-O-acetylated sialoglycoconjugates in activation of the alternate complement pathway in mammalian erythrocytes.

Abstract	Substitution of the -OH group at C-9 of sialic acid by an O-acetyl ester has been suggested to modify various biological phenomena that are regulated by sialic acids. Amongst them, enhancement of erythrocyte lysis by 9-O-acetylated sialic acid determinants through modulation of the alternate pathway of complement has been extensively studied on murine erythrocytes [1]. A variable expression of linkage specific 9-O-acetylated sialoglycoconjugates as defined by the lectinogenic epitope of Achatinin-H namely 9-O-acetylated sialic acid alpha2-->6Gal NAc was identified on rabbit, guinea pig, hamster, rat, mouse and human erythrocytes. This differential expression of linkage specific 9-O-acetylated sialoglycoconjugates strongly correlated with the susceptibility of mammalian erythrocytes to lysis by the alternate pathway of complement. Additionally, low levels of antibodies directed against O-acetylated sialic acids in these mammalian species suggested that these constitutively present determinants have low immunogenicity. Taken together, our results indicate that complement mediated hemolysis depends not simply upon the extent of surface 9-O-acetylated sialic acids present but more importantly upon the specific linkage.
Authors	V Sharma, M Chatterjee, G Sen, C A Kumar, C Mandal
Journal	Glycoconjugate journal (Glycoconj J) Vol. 17 Issue 12 Pg. 887-93 (Dec 2000) ISSN: 0282-0080 [Print] United States
PMID	11511813 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antibodies Glycoconjugates Lectins Membrane Glycoproteins Mucins Sialic Acids achatinin(H) 9-O-acetyl-N-acetylneuraminic acid
Topics	Acetylation Animals Antibodies (analysis, immunology) Carbohydrate Conformation Cattle Complement Activation Complement Pathway, Alternative Cricetinae Erythrocytes (metabolism) Glycoconjugates (chemistry, metabolism) Guinea Pigs Hemagglutination Hemagglutination Inhibition Tests Hemolysis Humans Lectins (metabolism) Membrane Glycoproteins (metabolism) Mice Mucins (metabolism) Rabbits Rats Sialic Acids (analysis, immunology, metabolism)

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