Minimal alterations in the HLA-B27-bound peptide repertoire induced upon infection of lymphoid cells with Salmonella typhimurium.

Abstract

OBJECTIVE:

To characterize putative changes in the HLA-B27-bound peptide repertoire following infection of lymphoid cells with Salmonella typhimurium, a bacterium known to trigger reactive arthritis in HLA-B27-positive individuals.

METHODS:

A protocol was developed for efficient large-scale infection of lymphoblastoid cell transfectants expressing HLA-B*2705. HLA-B27-bound peptide pools were isolated from noninfected and infected B*2705+ cells and comparatively analyzed by high-performance liquid chromatography. Peptide-containing chromatographic fractions from noninfected and infected cells were systematically compared by mass spectrometry (MS) to look for putative differences at the level of individual peptides.

RESULTS:

The presence of B*2705 did not influence S typhimurium invasion, since this was equally efficient in nontransfected or B27-transfected cells. The chromatographic profiles of B*2705-bound peptides from noninfected and infected cells were virtually identical. A total of 808 molecular species were compared by MS. Of these, 807 were present in both infected and noninfected cells. Only one molecular species from infected cells lacked a detectable counterpart in noninfected cells.

CONCLUSION:

Intracellular infection of lymphoid cells by S typhimurium induces minimal alterations in the HLA-B27-bound peptide repertoire. Minor changes detectable by cytotoxic T lymphocytes, but not easily amenable to direct biochemical analysis, are not ruled out.

• Authors: M Ramos, I Alvarez, F García-del-Portillo, J A López de Castro
• Journal: Arthritis and rheumatism (Arthritis Rheum) Vol. 44 Issue 7 Pg. 1677-88 (Jul 2001) ISSN: 0004-3591 [Print] United States
• PMID: 11465720 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HLA-B27 Antigen

Topics

• Arthritis, Reactive (immunology)
• Cell Line
• Chromatography, High Pressure Liquid
• HLA-B27 Antigen (analysis, immunology, metabolism)
• Humans
• Plasma Cells (cytology, microbiology)
• Salmonella Infections (immunology)
• Salmonella typhimurium
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization