Genetic changes in colorectal carcinoma tumors with liver metastases analyzed by comparative genomic hybridization and DNA ploidy.
Abstract | BACKGROUND: METHODS: The authors analyzed 35 primary tumors, including 16 primary tumors with liver metastasis, by using CGH and FCM. RESULTS: Increases in DNA copy numbers were detected in 6q (5 of 16 tumors), 7q (6 of 16 tumors), 8q (7 of 16 tumors), 9p (5 of 16 tumors), 13q (8 of 16 tumors), 20p (9 of 16 tumors), and 20q (15 of 16 tumors) in primary tumors with liver metastases. Decreases in DNA copy numbers were found in 17p (5 of 16 tumors), 18p (6 of 19 tumors), 18q (8 of 16 tumors), and 22q (5 of 16 tumors). In contrast, primary tumors without liver metastasis showed gains in chromosome arms 8q (2 of 19 tumors), 13q (2 of 19 tumors), 20p (6 of 19 tumors), and 20q (5 of 19 tumors); however, they showed no gains in 6q or 7q and showed losses in chromosome arms 17p (2 of 19 tumors), 18p (4 of 19 tumors), 18q (6 of 19 tumors), and 22q (5 of 19 tumors). There was a significant difference in the frequency of DNA copy number gains and losses in 6q (P < 0.05), 7q (P < 0.01), 8q (P < 0.05), 13q (P < 0.05), and 20q (P < 0.01), respectively, between primary tumors with and without liver metastases. The differences in the DNA index were not significant between the two groups of primary tumors. CONCLUSIONS: In liver metastases of primary tumors from patients with colorectal carcinoma, a correlation between DNA copy number aberrations and gains of chromosome arms 6q, 7q, 8q, 13q, and 20q is suggested.
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Authors | K Nakao, M Shibusawa, A Ishihara, H Yoshizawa, A Tsunoda, M Kusano, A Kurose, T Makita, K Sasaki |
Journal | Cancer
(Cancer)
Vol. 91
Issue 4
Pg. 721-6
(Feb 15 2001)
ISSN: 0008-543X [Print] United States |
PMID | 11241239
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2001 American Cancer Society. |
Chemical References |
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Topics |
- Chromosome Aberrations
- Chromosomes, Human, Pair 18
- Chromosomes, Human, Pair 20
- Colorectal Neoplasms
(genetics, pathology)
- DNA, Neoplasm
(analysis)
- Flow Cytometry
- Humans
- In Situ Hybridization, Fluorescence
- Liver Neoplasms
(genetics, secondary)
- Ploidies
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