Abstract | BACKGROUND/AIMS: METHODS: We used reverse transcription-polymerase chain reaction, in situ hybridization, ELISA and Western blot. RESULTS: Sixty-two of tested hepatocellular carcinomas were positive by reverse transcription-polymerase chain reaction. With in situ hybridization, a signal was found in every sample. In many cases, the signal was localized in cells labeled with an anti-smooth muscle alpka-actin antibody, while hepatocytes were mostly non-labeled. ELISA, performed in 15 pairs of hepatocellular carcinomas and surrounding livers, detected hepatocyte growth factor in every sample with wide variations. Hepatocellular carcinomas that had developed in non-cirrhotic livers contained essentially the same amount of hepatocyte growth factor as the matching non-tumoral liver. In cirrhotic livers, the hepatocyte growth factor content of the tumors was significantly lower than that of the surrounding cirrhotic livers. CONCLUSIONS:
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Authors | J Guirouilh, B Le Bail, L Boussarie, C Balabaud, P Bioulac-Sage, A Desmoulière, D Schuppan, J Rosenbaum |
Journal | Journal of hepatology
(J Hepatol)
Vol. 34
Issue 1
Pg. 78-83
(Jan 2001)
ISSN: 0168-8278 [Print] Netherlands |
PMID | 11211911
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Carcinoma, Hepatocellular
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Hepatocyte Growth Factor
(biosynthesis, genetics)
- Humans
- In Situ Hybridization
- Liver
(metabolism)
- Liver Neoplasms
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
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