Abstract |
Antibodies against Cryptosporidium parvum in the saliva and sera of three calves experimentally infected with this parasite were examined by an indirect immunofluorescence antibody test and immunoblotting. Salivary anti-C. parvum IgA antibody appeared on day 12 post-challenge and had a tendency to increase transiently between days 15 and 30 post-challenge. Salivary anti-C. parvum IgG antibody levels showed a gradual increase along with the change in IgA antibody levels during the infection. In contrast, serum anti-C. parvum IgA antibody levels showed only a slight increase between days 15 and 30 post-challenge. Serum anti-C. parvum IgG antibody levels rose on day 12 post-challenge and one calf maintained relatively high level up to the end of the experiment. In immunoblotting, an antigen with a molecular mass of 15 kDa was found to react strongly to salivary IgA antibody and a 27 kDa antigen to react to serum IgG antibody.
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Authors | A Toyoguchi, Y Omata, T Koyama, T Kamiyoshi, K Takeda, H Furuoka, Y Kobayashi, F Kakuda, R Maeda, T Matsui, A Saito, T Mikami |
Journal | The Journal of veterinary medical science
(J Vet Med Sci)
Vol. 62
Issue 11
Pg. 1231-4
(Nov 2000)
ISSN: 0916-7250 [Print] Japan |
PMID | 11129874
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Protozoan
- Immunoglobulin A
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Topics |
- Animals
- Animals, Newborn
- Antibodies, Protozoan
(analysis, blood)
- Antibody Formation
- Cattle
- Cattle Diseases
(blood, immunology, parasitology)
- Cryptosporidiosis
(blood, immunology, veterinary)
- Cryptosporidium parvum
(immunology)
- Fluorescent Antibody Technique, Indirect
- Immunoblotting
- Immunoglobulin A
(analysis, blood)
- Male
- Molecular Weight
- Saliva
(immunology)
- Time Factors
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