Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity.

Abstract

A new human colon cancer cell line (PMF-ko14) derived from a peritoneal disseminated tumor has been established and maintained for over 25 months. In tissue culture, the cells grew in a mainly monolayered sheet with a population doubling time of about 27 h. Chromosome counts at the 60th passage ranged from 79 to 84 with a modal number of 83. Flow cytometry of the cell surface antigen expression indicated that CD49b, CD29, carcinoembryonic antigen (CEA), sialyl Lewis a (sLea), and CD49c were positive in more than 70% of the cells. The nude mouse xenograft models indicated are: subcutaneous or intraperitoneal injection model, spleen injection-liver metastasis model, and orthotopic implantation-spontaneous metastasis model. As PMF-ko14 has highly metastatic activity it should prove to be a useful tool for research in biological behavior of metastatic colon cancer.

• Authors: K Okazaki, Y Nakayama, K Shibao, K Hirata, T Sako, N Nagata, Y Kuroda, H Itoh
• Journal: International journal of oncology (Int J Oncol) Vol. 17 Issue 1 Pg. 39-45 (Jul 2000) ISSN: 1019-6439 [Print] Greece
• PMID: 10853016 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD

Topics

• Animals
• Antigens, CD (analysis)
• Cell Culture Techniques (methods)
• Cell Division
• Chromosome Banding
• Colonic Neoplasms (genetics, pathology)
• Flow Cytometry
• Humans
• Karyotyping
• Kinetics
• Liver Neoplasms (pathology, secondary)
• Mice
• Mice, Nude
• Neoplasm Metastasis
• Peritoneal Neoplasms (pathology, secondary)
• Transplantation, Heterologous
• Tumor Cells, Cultured