Abstract | OBJECTIVE: DESIGN: Randomized controlled trial. SUBJECTS: Piglets (0 to 3 days old). INTERVENTION: Carotid snares and arterial and venous catheters were placed under 1.5% isoflurane anesthesia. In Experiment 1, piglets were randomly assigned to receive either 3 mg/kg desmethyl tirilazad or vehicle at -15 and 90 mins. In Experiment 2, piglets were randomly assigned to receive either 20 mg/kg desmethyl tirilazad at -15 mins followed by 8 mg/kg/hr for 90 mins or 100 mg/kg deferoxamine at -15 mins or vehicle. At time 0, both carotid arteries were clamped and blood was withdrawn to reduce the blood pressure to two-thirds normal. At 15 mins, inspired oxygen was reduced to 6%. At 30 mins, the carotid snares were released, the withdrawn blood was reinfused, and the oxygen was switched to 100%. On the third day after the hypoxic ischemic injury, the animals were killed by perfusing their brains with 10% formalin. We tested the timing of lipid peroxidation and inhibition of lipid peroxidation by these agents by freezing the brains of a subset of pigs in liquid nitrogen. MEASUREMENTS: MAIN RESULTS: CONCLUSIONS:
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Authors | Y Feng, M H LeBlanc, E B LeBlanc, C C Parker, J D Fratkin, X B Qian, D M Patel, M Huang, E E Smith, P J Vig |
Journal | Critical care medicine
(Crit Care Med)
Vol. 28
Issue 5
Pg. 1431-8
(May 2000)
ISSN: 0090-3493 [Print] United States |
PMID | 10834691
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Pregnatrienes
- Reactive Oxygen Species
- U 74389F
- Deferoxamine
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Topics |
- Animals
- Animals, Newborn
- Antioxidants
(pharmacology)
- Asphyxia Neonatorum
(pathology, physiopathology)
- Brain
(blood supply, pathology)
- Deferoxamine
(pharmacology)
- Dose-Response Relationship, Drug
- Humans
- Hypoxia, Brain
(pathology, physiopathology)
- Infant, Newborn
- Lipid Peroxidation
(drug effects, physiology)
- Neurologic Examination
(drug effects)
- Pregnatrienes
(pharmacology)
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(pathology, physiopathology)
- Swine
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