[Quantitation of human immunodeficiency virus type 1 RNA by ultrasensitive method in the analysis of samples with low viral load].

Abstract

With the introduction of combination therapy to the patients with Human Immunodeficiency Virus Type 1(HIV-1) infection, HIV-1 RNA levels in plasma have been reduced, frequently to less than limit of quantitation(400 copies/ml). To achieve enhanced sensitivity, a modified specimen preparation procedure that allows the quantitation of plasma HIV-1 RNA levels as low as 50 copies/ml was developed. Of 67 samples with less than 400 copies/ml of HIV-1 RNA by standard method, 39(58.2%) were not detected by the "ultrasensitive" method. Among 5 samples obtained from patients who were treated with 2 nucleoside reverse transcriptase inhibitors, 4 samples still had detectable copies of HIV-1 RNA. Those suggest that the ultrasensitive assay for HIV-1 RNA has advantage for clinical practice.

• Authors: Y Nishida, K Moriya, K Nagaizumi, H Inaba, K Amano, H Fukue, Y Yamamoto, M Arai, K Fukutake
• Journal: Rinsho byori. The Japanese journal of clinical pathology (Rinsho Byori) Vol. 48 Issue 3 Pg. 282-3 (Mar 2000) ISSN: 0047-1860 [Print] Japan
• PMID: 10804838 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Viral

Topics

• HIV Infections (blood)
• HIV-1 (genetics, isolation & purification)
• Humans
• Methods
• RNA, Viral (blood)
• Sensitivity and Specificity