High expression of KMT2D is a promising biomarker for poor gastric cancer prognosis.

Abstract

OBJECTIVE:

To investigate the expression of histone lysine N-methyltransferase 2D (KMT2D) in gastric cancer patients and its relationship with the prognosis.

METHODS:

A total of 126 gastric cancer patients admitted to Hubei Provincial Hospital of TCM from January 2014 to June 2017 were selected as the research subjects, and patients' clinical data were analyzed retrospectively. First, the KMT2D mRNA or protein expression in the patient's tissue was detected using quantitative real-time PCR or immunohistochemistry. Afterwards, the relationship between the KMT2D protein expression and the prognosis of patients was analyzed using a Kaplan-Meier curve. Also, the predictive value of the KMT2D mRNA and protein expression for the prognosis and death rate of gastric cancer patients was evaluated using a receiver operating characteristic curve. Finally, the risk factors for poor prognosis and death of the gastric cancer patients were analyzed using a Cox regression analysis.

RESULTS:

Overall, the KMT2D mRNA expression level and positive rate of protein expression in the gastric cancer tissues were significantly higher than that in paracancerous tissues (P<0.05). A positive expression of the KMT2D protein in gastric cancer tissues was correlated with the following factors in patients: age ≥60 years, tumor differentiation degree, TNM stage III-IV, lymph node metastasis, depth of invasion T3-T4, distant metastasis and high serum carbohydrate antigen 19-9 (CA19-9) levels (P<0.05). Also, the 5-year overall survival and progression-free survival of gastric cancer patients with a positive KMT2D expression were lower than those with negative KMT2D expressions (P<0.05). The resulting areas under the curve for predicting the prognosis and death of gastric cancer patients with the KMT2D mRNA and protein expression were 0.823 and 0.645, respectively. In addition, tumor maximum diameter >5 cm, poor differentiation, TNM stage III-IV, lymph node metastasis, high serum CA19-9 level, KMT2D mRNA expression ≥1.48 and KMT2D protein positive expression were risk factors affecting the prognosis and death of gastric cancer patients (P<0.05).

CONCLUSION:

KMT2D is highly expressed in gastric cancer tissue and it is expected to be a potential biomarker for predicting the poor prognosis of gastric cancer patients.

• Authors: Tianjiao Yi, Lizhong Peng, Pan Li, Yun He, Junjun Tian
• Journal: American journal of translational research (Am J Transl Res) Vol. 15 Issue 3 Pg. 1964-1972 ( 2023) ISSN: 1943-8141 [Print] United States
• PMID: 37056814 (Publication Type: Journal Article)
• Copyright: AJTR Copyright © 2023.