EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis.

Abstract

BACKGROUND:

EC-18, a synthetic monoacetyldiaglyceride, exhibits protective effects against lung inflammation, allergic asthma, and abdominal sepsis. However, there have been no investigations to determine whether EC-18 has preventive potential in autoimmune diseases, especially rheumatoid arthritis (RA).

METHODS:

To investigate the efficacy of EC-18 on the development of RA, EC-18 was administered in a collagen-induced arthritis (CIA) murine model and disease severity and the level of inflammatory cytokines in the joint were investigated. The effect of EC-18 on the inflammation-related factors was investigated by flow cytometry, ELISA, western blot, and real-time PCR in splenocytes from mice and in peripheral blood mononuclear cells from healthy and patients with RA. The effect of EC-18 on osteoclastogenesis was investigated.

RESULTS:

EC-18 effectively reduced the clinical and histological severity of arthritis, similar to Janus kinase inhibitors include tofacitinib and baricitinib, in CIA. Furthermore, EC-18 exhibited a synergistic effect with methotrexate in preventing CIA. Treatment with EC-18 effectively reduced the production of inflammatory cytokines in immune cells and osteoclast differentiation in mice and patients with RA.

CONCLUSION:

These results suggest that EC-18 may be an effective strategy for RA.

• Authors: Jin-Sil Park, Seung Cheon Yang, Ha Yeon Jeong, Seon-Young Lee, Jun-Geol Ryu, Jeong Won Choi, Hye Yeon Kang, Sung-Min Kim, Sun-Hee Hwang, Mi-La Cho, Sung-Hwan Park
• Journal: Arthritis research & therapy (Arthritis Res Ther) Vol. 24 Issue 1 Pg. 254 (11 17 2022) ISSN: 1478-6362 [Electronic] England
• PMID: 36397156 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022. The Author(s).

Chemical References

• Cytokines

Topics

• Mice
• Animals
• Osteogenesis
• Cytokines (pharmacology)
• Leukocytes, Mononuclear (pathology)
• Arthritis, Experimental
• Arthritis, Rheumatoid (drug therapy)