Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: Here, we aim to explore the potential mechanism of QHJD in relieving acute gouty arthritis. MATERIALS AND METHODS: Acute gouty arthritis model was established by injecting monosodium urate (MSU) suspension into knee joint. The pathological state of synovial tissue in each group was evaluated by hematoxylin- eosin (HE) staining. The level of TNF-α, IL-6, and IL-1β were detected by enzyme-linked immunosorbent assay (ELISA). qRT-PCR was used to detect the mRNA expression of NLRP3, ATG5, ATG7, PI3K, AKT, and mTOR. The protein expression of LC3II/I, p62, ULK1, P-ULK1, Beclin-1, PI3K, AKT, mTOR, P-PI3K, P-AKT, and P-mTOR were detected by Western blot. RESULTS: (1) The level of autophagy protein ( mRNA) was significantly up-regulated in QHJD group and rapamycin, while the expression of autophagy protein ( mRNA) was significantly downregulated in the 3-methyladenoenoic acid (3 MA) group; (2) QHJD and rapamycin significantly inhibited PI3K/AKT/mTOR pathway, while 3 MA group activated this pathway. (3) It was worth noting that after treatment with QHJD and rapamycin, the inflammatory pathological state of AGA synovial tissue was significantly reduced with the activation of the autophagy gene in knee synovial tissue, and the inhibition of PI3K/AKT/mTOR pathway. CONCLUSIONS: This research revealed that QHJD activates autophagy by inhibiting PI3K/AKT/mTOR pathway, thereby relieving acute gouty arthritis.
|
Authors | Peiyu Liu, Yang Xu, Jiaxue Ye, Jingrui Tan, Jie Hou, Yazhuo Wang, Jianwei Li, Weizhen Cui, Shiyuan Wang, Qingyang Zhao |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 302
Issue Pt A
Pg. 115875
(Feb 10 2023)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 36328206
(Publication Type: Journal Article)
|
Copyright | Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Proto-Oncogene Proteins c-akt
- Phosphatidylinositol 3-Kinases
- TOR Serine-Threonine Kinases
- Sirolimus
- RNA, Messenger
- MTOR protein, human
|
Topics |
- Humans
- Proto-Oncogene Proteins c-akt
(metabolism)
- Phosphatidylinositol 3-Kinases
(metabolism)
- Arthritis, Gouty
(chemically induced, drug therapy, metabolism)
- TOR Serine-Threonine Kinases
(metabolism)
- Signal Transduction
- Autophagy
- Sirolimus
- RNA, Messenger
|