Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging: Diagnostic Pathways and Metabolites for Renal Tumor Entities.
Abstract | BACKGROUND: Correct tumor subtyping of primary renal tumors is essential for treatment decision in daily routine. Most of the tumors can be classified based on morphology alone. Nevertheless, some diagnoses are difficult, and further investigations are needed for correct tumor subtyping. Besides histochemical investigations, high-mass-resolution matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can detect new diagnostic biomarkers and hence improve the diagnostic. PATIENTS AND METHODS: RESULTS: We discriminated the four histological subtypes (ccRCC, pRCC, chRCC, and rO) and established the subtype-specific pathways and metabolic profiles. rO showed an enrichment of pentose phosphate, taurine and hypotaurine, glycerophospholipid, amino sugar and nucleotide sugar, fructose and mannose, glycine, serine, and threonine pathways. ChRCC is defined by enriched pathways including the amino sugar and nucleotide sugar, fructose and mannose, glycerophospholipid, taurine and hypotaurine, glycine, serine, and threonine pathways. Pyrimidine, amino sugar and nucleotide sugar, glycerophospholipids, and glutathione pathways are enriched in ccRCC. Furthermore, we detected enriched phosphatidylinositol and glycerophospholipid pathways in pRCC. CONCLUSION: In summary, we performed a classification system with a mean accuracy in tumor discrimination of 85.13%. Furthermore, we detected tumor-specific biomarkers for the four most common primary renal tumors by MALDI-MSI. This method is a useful tool in differential diagnosis and biomarker detection.
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Authors | Franziska Erlmeier, Na Sun, Jian Shen, Annette Feuchtinger, Achim Buck, Verena M Prade, Thomas Kunzke, Peter Schraml, Holger Moch, Michael Autenrieth, Wilko Weichert, Arndt Hartmann, Axel Walch |
Journal | Oncology
(Oncology)
Vol. 101
Issue 2
Pg. 126-133
( 2023)
ISSN: 1423-0232 [Electronic] Switzerland |
PMID | 36198279
(Publication Type: Journal Article)
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Copyright | © 2022 S. Karger AG, Basel. |
Chemical References |
- hypotaurine
- Mannose
- Taurine
- Biomarkers, Tumor
- Transcription Factors
- Amino Sugars
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Topics |
- Humans
- Carcinoma, Renal Cell
(diagnostic imaging, metabolism)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Mannose
- Kidney Neoplasms
(metabolism)
- Taurine
- Biomarkers, Tumor
- Transcription Factors
- Amino Sugars
- Lasers
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