Biodegradable Hollow-Structured Nanozymes Modulate Phenotypic Polarization of Macrophages and Relieve Hypoxia for Treatment of Osteoarthritis.

Abstract

Nanozymes are widely applied for treating various major diseases, including neurological diseases and tumors. However, the biodegradability of nanozymes remains a great challenge, which hinders their further clinical translation. Based on the microenvironment of osteoarthritis (OA), a representative pH-responsive biodegradable hollow-structured manganese Prussian blue nanozyme (HMPBzyme) is designed and applied for treatment of OA. HMPBzyme with good pH-responsive biodegradability, biocompatibility, and multi-enzyme activities is constructed by bovine serum albumin bubbles as a template-mediated biomineralization strategy. HMPBzyme suppresses hypoxia-inducible factor-1α (HIF-1α) expression and decreases reactive oxygen species (ROS) level in the in vitro experiment. Furthermore, HMPBzyme markedly suppresses the expression of ROS and alleviates the degeneration of cartilage in OA rat models. The results indicate that the biodegradable HMPBzyme inhibits oxidative damage and relieves hypoxia synergistically to suppress inflammation and promote the anabolism of cartilage extracellular matrix by protecting mitochondrial function and down-regulating the expression of HIF-1α, which modulates the phenotypic conversion of macrophages from pro-inflammatory M1 subtype to anti-inflammatory M2 subtype for OA treatment. This research lays a solid foundation for the design, construction, and biomedical application of biodegradable nanozymes and promotes the application of nanozymes in biomedicine.

• Authors: Hao Xiong, Yongzheng Zhao, Qinyuan Xu, Xue Xie, Jianrong Wu, Bing Hu, Shuai Chen, Xiaojun Cai, Yuanyi Zheng, Cunyi Fan
• Journal: Small (Weinheim an der Bergstrasse, Germany) (Small) Vol. 18 Issue 32 Pg. e2203240 (08 2022) ISSN: 1613-6829 [Electronic] Germany
• PMID: 35843877 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022 The Authors. Small published by Wiley-VCH GmbH.

Chemical References

• Reactive Oxygen Species

Topics

• Animals
• Hypoxia (metabolism, pathology)
• Inflammation (pathology)
• Macrophages (metabolism)
• Osteoarthritis (drug therapy)
• Rats
• Reactive Oxygen Species (metabolism)