Upregulation of IFNɣ-mediated chemokines dominate the immune transcriptome of muscle-invasive urothelial carcinoma.

Abstract

Tumor inflammation is prognostically significant in high-grade muscle-invasive bladder cancer (MIBC). However, the underlying mechanisms remain elusive. To identify inflammation-associated immune gene expression patterns, we performed transcriptomic profiling of 40 MIBC archival tumors using the NanoString nCounter Human v.1.1 PanCancer Panel. Findings were validated using the TCGA MIBC dataset. Unsupervised and supervised clustering identified a distinctive immune-related gene expression profile for inflammation, characterized by significant upregulation of 149 genes, particularly chemokines, a subset of which also had potential prognostic utility. Some of the most enriched biological processes were lymphocyte activation and proliferation, leukocyte adhesion and migration, antigen processing and presentation and cellular response to IFN-γ. Upregulation of numerous IFN-γ-inducible chemokines, class II MHC molecules and immune checkpoint genes was detected as part of the complex immune response to MIBC. Further, B-cell markers linked to tertiary lymphoid structures were upregulated, which in turn is predictive of tumor response to immunotherapy and favorable outcome. Our findings of both an overall activated immune profıle and immunosuppressive microenvironment provide novel insights into the complex immune milieu of MIBC with inflammation and supports its clinical significance for predicting prognosis and immunotherapeutic responsiveness, which warrants further investigation. This may open novel opportunities to identify mechanisms for developing new immunotherapeutic strategies.

• Authors: Ekaterina Olkhov-Mitsel, Anjelica Hodgson, Stan K Liu, Danny Vesprini, Jane Bayani, John M S Bartlett, Bin Xu, Michelle R Downes
• Journal: Scientific reports (Sci Rep) Vol. 12 Issue 1 Pg. 716 (01 13 2022) ISSN: 2045-2322 [Electronic] England
• PMID: 35027623 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022. The Author(s).

Chemical References

• Chemokines
• Interferon-gamma

Topics

• Adult
• Aged
• Aged, 80 and over
• Chemokines (immunology, metabolism)
• Female
• Gene Expression Profiling
• Humans
• Immunotherapy
• Inflammation
• Interferon-gamma (genetics, immunology, metabolism)
• Lymphocyte Activation
• Male
• Middle Aged
• Neoplasm Invasiveness
• Transcriptome
• Tumor Microenvironment (immunology)
• Up-Regulation (genetics)
• Urinary Bladder Neoplasms (genetics, immunology, pathology, therapy)