Abstract |
Oxidative stress plays an important role in the pathophysiology of acute kidney injury (AKI). Previously, we reported that vanin-1, which is involved in oxidative stress, is associated with renal tubular injury. This study was aimed to determine whether urinary vanin-1 is a biomarker for the early diagnosis of AKI in two experimental models: in vivo and in vitro. In a rat model of AKI, ischemic AKI was induced in uninephrectomized rats by clamping the left renal artery for 45 min and then reperfusing the kidney. On Day 1 after renal ischemia/reperfusion (I/R), serum creatinine (SCr) in I/R rats was higher than in sham-operated rats, but this did not reach significance. Urinary N-acetyl-β-D- glucosaminidase (NAG) exhibited a significant increase but decreased on Day 2 in I/R rats. In contrast, urinary vanin-1 significantly increased on Day 1 and remained at a significant high level on Day 2 in I/R rats. Renal vanin-1 protein decreased on Days 1 and 3. In line with these findings, immunofluorescence staining demonstrated that vanin-1 was attenuated in the renal proximal tubules of I/R rats. Our in vitro results confirmed that the supernatant from HK-2 cells under hypoxia/reoxygenation included significantly higher levels of vanin-1 as well as KIM-1 and NGAL. In conclusion, our results suggest that urinary vanin-1 might be a potential novel biomarker of AKI induced by I/R.
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Authors | Keiko Hosohata, Denan Jin, Shinji Takai |
Journal | International journal of molecular sciences
(Int J Mol Sci)
Vol. 22
Issue 21
(Oct 23 2021)
ISSN: 1422-0067 [Electronic] Switzerland |
PMID | 34768879
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- Creatinine
- Hexosaminidases
- Amidohydrolases
- Vnn1 protein, rat
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Topics |
- Acute Kidney Injury
(metabolism, physiopathology, urine)
- Amidohydrolases
(metabolism, urine)
- Animals
- Biomarkers
(urine)
- Creatinine
(analysis, blood)
- Early Diagnosis
- Hexosaminidases
(metabolism, urine)
- Ischemia
(metabolism)
- Kidney
(metabolism)
- Male
- Oxidative Stress
(physiology)
- Rats
- Rats, Sprague-Dawley
- Reperfusion
- Reperfusion Injury
(metabolism, physiopathology, urine)
- Urinary Tract
(metabolism)
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