Abstract | CONTEXT: OBJECTIVE: We investigated the effects of 18β-GA on MA104 cells infected with rotavirus (RV) and its potential mechanism of action. MATERIALS AND METHODS: Cell Counting Kit-8 was used to assess tissue culture infective dose 50 (TCID50) and 50% cellular cytotoxicity (CC50) concentration. MA104 cells infected with RV SA11 were treated with 18β-GA (1, 2, 4, and 8 μg/mL, respectively). Cytopathic effects were observed. The virus inhibition rate, concentration for 50% of maximal effect (EC50), and selection index (SI) were calculated. Cell cycle, cell apoptosis, and mRNA and protein expression related to the Fas/FasL pathway were detected. RESULTS: TCID50 of RV SA11 was 10-4.47/100 µL; the CC50 of 18β-GA on MA104 cells was 86.92 µg/mL. 18β-GA showed significant antiviral activity; EC50 was 3.14 μg/mL, and SI was 27.68. The ratio of MA104 cells infected with RV SA11 in the G0/G1 phase and the G2/M phase decreased and increased, respectively, after 18β-GA treatment. 18β-GA significantly induced apoptosis in the infected cells. Furthermore, after 18β-GA treatment, the mRNA and protein expression levels of Fas, FasL, caspase 3, and Bcl-2 decreased, whereas the expression levels of Bax increased. DISCUSSION AND CONCLUSIONS: The study demonstrates that 18β-GA may be a promising candidate for the treatment of RV SA11 infection and provides theoretical support for the clinical development of glycyrrhizic acid compounds for the treatment of RV infection.
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Authors | Xiaoyan Wang, Fang Xie, Xiaofeng Zhou, Ting Chen, Ye Xue, Wei Wang |
Journal | Pharmaceutical biology
(Pharm Biol)
Vol. 59
Issue 1
Pg. 1098-1105
(Dec 2021)
ISSN: 1744-5116 [Electronic] England |
PMID | 34411493
(Publication Type: Journal Article, Retracted Publication)
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Chemical References |
- Antiviral Agents
- Fas Ligand Protein
- RNA, Messenger
- fas Receptor
- 18alpha-glycyrrhetinic acid
- Glycyrrhetinic Acid
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Topics |
- Animals
- Antiviral Agents
(administration & dosage, pharmacology)
- Apoptosis
(drug effects)
- Cell Cycle
(drug effects)
- Cell Line
- Dose-Response Relationship, Drug
- Fas Ligand Protein
(metabolism)
- Glycyrrhetinic Acid
(administration & dosage, analogs & derivatives, pharmacology)
- Haplorhini
- RNA, Messenger
(metabolism)
- Rotavirus
(drug effects)
- Rotavirus Infections
(drug therapy, virology)
- fas Receptor
(metabolism)
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