Diagnostic significance of circulating miR-485-5p in patients with lupus nephritis and its predictive value evaluation for the clinical outcomes.

Abstract

BACKGROUND:

Lupus nephritis (LN) is one of the main risk factors contributing to morbidity and mortality of systemic lupus erythematosus (SLE). This study aimed to investigate the potential role of miR-485-5p in human LN.

METHODS:

Quantitative real-time polymerase chain reaction was used for the measurement of miR-485-5p levels. The levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) in serum were determined by enzyme-linked immunosorbent assay. The diagnostic role of miR-485-5p in LN was evaluated by the receiver operating characteristic (ROC) curve. The impact of miR-485-5p on end-stage renal disease (ESRD) was compared by Kaplan-Meier analysis and Cox regression analysis. The target gene was determined by a dual-luciferase reporter assay system.

RESULTS:

MiR-485-5p was highly expressed in SLE and LN patients compared with the healthy controls, and LN patients had the highest level of miR-485-5p. The expression level of miR-485-5p in active LN patients was significantly increased compared with that in nonactive cases. MiR-485-5p expression showed a positive correlation with the levels of estimated glomerular filtration rate, serum creatinine, proteinuria, SLE disease activity index score, and inflammatory cytokines. The ROC analysis results indicated that serum miR-485-5p was a promising biomarker for the early diagnosis of LN, and it can distinguish active LN patients from nonactive ones. Phosphatase and tensin homolog was a direct target of miR-485-5p, and negatively associated with serum miR-485-5p levels. More ESRD events were observed in cases with high miR-485-5p expression, miR-485-5p was an independent factor for the risk of ESRD in LN patients.

CONCLUSION:

Serum miR-485-5p might be a novel promising diagnostic marker for LN and has potential predictive value for ESRD risk in LN patients.

• Authors: Qinghua Wu, Yanan Qin, Mei Shi, Liping Yan
• Journal: Journal of the Chinese Medical Association : JCMA (J Chin Med Assoc) Vol. 84 Issue 5 Pg. 491-497 (05 01 2021) ISSN: 1728-7731 [Electronic] Netherlands
• PMID: 33742995 (Publication Type: Journal Article)
• Copyright: Copyright © 2021, the Chinese Medical Association.

Chemical References

• Biomarkers
• MIRN485 microRNA, human
• MicroRNAs

Topics

• Adolescent
• Adult
• Biomarkers
• Female
• Humans
• Lupus Nephritis (diagnosis)
• Male
• MicroRNAs (blood)
• Middle Aged
• Outcome Assessment, Health Care
• Prognosis
• Young Adult