Abstract |
Cholestasis can induce liver fibrosis and cirrhosis. Apigenin has anti-oxidant and anti-inflammatory effects. Herein, we determined whether apigenin can protect mice against cholestasis. In vitro, apigenin protected TFK-1 cells (a human bile duct cancer cell line) against H2O2-induced ROS generation and inhibited transforming growth factor-β-activated collagen type 1 alpha 1 and α-smooth muscle actin in LX2 cells (a human hepatic stellate cell line). In vivo, cholestatic mice induced by 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) were treated with apigenin. Apigenin potently blocked DDC-induced gallbladder atrophy and associated liver injury, fibrosis and collagen accumulation. Moreover, apigenin relieved the DDC-caused abnormality of bile acid metabolism and restored the balance between bile secretion and excretion by regulating the farnesoid X receptor signaling pathway. Furthermore, apigenin reduced inflammation or oxidative stress in the liver by blocking the DDC-activated Toll-like receptor 4, nuclear factor κB and tumor necrosis factor α, or DDC-suppressed superoxidase dismutase 1/2, catalase and glutathione peroxidase. Taken together, apigenin improves DDC-induced cholestasis by reducing inflammation and oxidative damage and improving bile acid metabolism, indicating its potential application for cholestasis treatment.
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Authors | Shihong Zheng, Peichang Cao, Zequn Yin, Xuerui Wang, Yuanli Chen, Maoyun Yu, Baocai Xu, Chenzhong Liao, Yajun Duan, Shuang Zhang, Jihong Han, Xiaoxiao Yang |
Journal | Food & function
(Food Funct)
Vol. 12
Issue 5
Pg. 2323-2334
(Mar 15 2021)
ISSN: 2042-650X [Electronic] England |
PMID | 33620063
(Publication Type: Journal Article)
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Chemical References |
- 3,5-diethoxycarbonyl-1,4-dihydrocollidine
- Bile Acids and Salts
- Protective Agents
- Pyridines
- Apigenin
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Topics |
- Animals
- Apigenin
(pharmacology)
- Bile Acids and Salts
(metabolism)
- Cell Line, Tumor
- Cholestasis
(chemically induced, metabolism)
- Humans
- Liver
(drug effects)
- Liver Cirrhosis
(metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- Oxidative Stress
(drug effects)
- Protective Agents
(pharmacology)
- Pyridines
(adverse effects)
- Signal Transduction
(drug effects)
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