Effect of micro
ribonucleic acid (miR)-30 on the proliferation of trophoblasts in
preeclampsia (PE) rats through the
mitogen-activated protein kinase (MAPK)/
extracellular signal-regulated kinase (ERK) pathway was studied. The miR-30 mimic was transfected into the trophoblast HTR8/SVNEO cell lines. The effects of expression level of miR-30 on the proliferation and
hypoxia-induced apoptosis of HTR8/SVNEO cells were detected via methyl thiazolyl tetrazolium (MTT) assay and
Annexin V/
propidium iodide staining, respectively, using the flow cytometer. A total of 30 pregnant Sprague-Dawley rats were randomly divided into control group (CTL group, n=10), PE rat group (PE group, n=10) and PE + miR-30 Mimic group (PE+agomiR-30 group, n=10) using a random number table. The
protein expression levels of phosphorylated ERK (p-ERK)1/2, ERK1/2,
proliferating cell nuclear antigen (
PCNA) and
tubulin were determined using western blot analysis, and the
mRNA expression level of ERK1/2 was detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression level of
PCNA in tissues was detected via immunohistochemistry. The results of MTT assay showed that the proliferation of HTR8/SVNEO cells significantly declined in hypoxic environment, while miR-30 promoted the proliferation of HTR8/SVNEO cells and alleviated the
hypoxia-induced inhibition on cell proliferation. It was found that the trophoblast apoptosis rate was increased in
hypoxia group compared with that in CTL group, while it was significantly decreased in miR-30 Mimic group compared with that in
hypoxia group. PE group had obviously decreased p-ERK and
PCNA expression levels as well as p-ERK/ERK ratio in placental tissues compared with CTL group, while PE+agomiR-30 group had an obviously increased expression level of
PCNA as well as p-ERK/ERK ratio in placental tissues compared with PE group. MiR-30 activates the MAPK/ERK signaling pathway and increases the expression level of
PCNA through raising the p-ERK level and p-ERK/ERK ratio, thereby inhibiting cell apoptosis and promoting cell proliferation.