Glomerular endothelial cells and podocytes can express CD80 in patients with minimal change disease during relapse.

Abstract

BACKGROUND:

Urinary CD80 has emerged as potential biomarker in idiopathic nephrotic syndrome (INS). However, its cellular source remains controversial. The aim of the study was to assess whether CD80 is truly expressed by glomerular cells in INS patients during relapse and in the LPS mouse model of podocyte injury.

METHODS:

The presence of CD80 in glomeruli was evaluated by combining immunostaining, immunogold labeling, and in situ hybridization techniques.

RESULTS:

CD80 was present along the surface of glomerular endothelial cells (GEC) and rarely in podocytes in six of nine minimal change disease (MCD) patients in relapse, two of eleven patients with focal segmental glomerulosclerosis in relapse, and absent in controls. In mice, CD80 was upregulated at mRNA and protein level in GEC and podocytes, in a similar pattern to that seen in MCD patients.

CONCLUSIONS:

Glomerular endothelial cells and podocytes can express CD80 in patients with MCD during relapse. A better understanding of the role of CD80 in glomerular cells may provide further insights into the mechanisms of proteinuria in INS.

• Authors: Gabriel Cara-Fuentes, Madhusudan Venkatareddy, Rakesh Verma, Alfons Segarra, Audrey C Cleuren, Alfonso Martínez-Ramos, Richard J Johnson, Puneet Garg
• Journal: Pediatric nephrology (Berlin, Germany) (Pediatr Nephrol) Vol. 35 Issue 10 Pg. 1887-1896 (10 2020) ISSN: 1432-198X [Electronic] Germany
• PMID: 32399663 (Publication Type: Journal Article, Observational Study, Research Support, N.I.H., Extramural)

Chemical References

• B7-1 Antigen
• Biomarkers
• CD80 protein, human

Topics

• Adult
• Animals
• B7-1 Antigen (metabolism, urine)
• Biomarkers (metabolism, urine)
• Biopsy
• Endothelial Cells (metabolism, ultrastructure)
• Female
• Glomerulosclerosis, Focal Segmental (diagnosis, pathology, urine)
• Humans
• Kidney Glomerulus (cytology, pathology, ultrastructure)
• Male
• Mice
• Microscopy, Electron, Transmission
• Middle Aged
• Nephrosis, Lipoid (diagnosis, pathology, urine)
• Podocytes (metabolism, ultrastructure)
• Recurrence
• Young Adult