Abstract | BACKGROUND: METHODS: Twelve newborn C57BL/6 J male mice were subcutaneously injected with monosodium glutamate ( MSG) to induce obesity on a conventional diet. Six mice were also administered 5% FOS via drinking water from 10 weeks of age. At 18 weeks, histological characteristics of the liver and epididymal fat were compared between the groups. Hepatic mRNA expression of lipid metabolism enzymes and SCFA in feces and sera were measured. RESULTS: Hepatic steatosis, inflammatory cell infiltration, and hepatocyte ballooning in the liver and increased hepatic mRNA expression of fatty acid synthase and glycerol-3-phosphate acyltransferase were observed in the MSG-treated mice. FOS treatment improved the liver pathology and blunted the increases in the mRNA expression levels of lipid metabolism enzymes. In addition, FOS inhibited adipocyte enlargement and formation of crown-like structures and reduced the M1 macrophage frequency in the epididymal fat of the MSG mice (39.4% ± 3.0% vs. 22.8% ± 0.7%; P = 0.001). FOS increased not only the fecal concentrations of n- butyric acid (0.04 ± 0.01 vs. 0.38 ± 0.14 mg/g, P = 0.02), propionic acid (0.09 ± 0.03 vs. 0.42 ± 0.16 mg/g, P = 0.02), and acetic acid (0.65 ± 0.16 vs. 1.48 ± 0.29 mg/g, P = 0.03) but also the serum concentration of propionic acid (3.9 ± 0.5 vs. 8.2 ± 0.5 μmol/L, P = 0.001). CONCLUSIONS:
|
Authors | Atsuko Takai, Kentaro Kikuchi, Mayuko Ichimura, Koichi Tsuneyama, Yuki Moritoki, Kotaro Matsumoto, Hiromichi Tsunashima, Takeshi Onda, Noriyuki Kuniyoshi, Tomoyuki Nariyama, Sho Ohyatsu, Juri Kubota, Kozue Nagumo, Shinpei Sato, Masumi Hara, Hiroshi Miyakawa |
Journal | BMC gastroenterology
(BMC Gastroenterol)
Vol. 20
Issue 1
Pg. 46
(Feb 27 2020)
ISSN: 1471-230X [Electronic] England |
PMID | 32103741
(Publication Type: Journal Article)
|
Chemical References |
- Fatty Acids, Volatile
- Oligosaccharides
|
Topics |
- Animals
- Disease Models, Animal
- Fatty Acids, Volatile
(metabolism)
- Fruit
- Male
- Mice
- Mice, Inbred C57BL
- Non-alcoholic Fatty Liver Disease
(diet therapy)
- Obesity, Abdominal
(diet therapy)
- Oligosaccharides
(administration & dosage, pharmacology)
|