Netrin-1 and its receptor Unc5b as markers of periodontal disease.

Abstract

OBJECTIVES:

The aim of this present study was to evaluate the levels of Netrin-1 and Unc5b in periodontal health and disease.

BACKGROUND:

Netrin-1, acting via its receptor UNC5b, regulates the inflammatory response and takes apart in bone destructive diseases.

METHODS:

Samples of gingival crevicular fluid (GCF), whole saliva, and serum were taken from systemically healthy, nonsmoking 20 periodontitis , 20 gingivitis patients, and 20 periodontally healthy subjects at baseline and 4 weeks after nonsurgical periodontal treatment (NSPT). Whole-mouth and site-specific clinical periodontal parameters were recorded. Netrin-1 and Unc5b levels were measured by enzyme-linked immunosorbent assay. Data were analyzed by nonparametric tests.

RESULTS:

Total amount of Netrin-1 in GCF was significantly higher in periodontitis than the others, and the levels were significantly reduced after NSPT. Salivary and serum concentrations of Netrin-1 were significantly different among the study groups (P = .000), and NSPT significantly increased the concentration levels of both salivary and serum Netrin-1 (P < .05). Healthy subjects had significantly lower GCF (P = .001) and conversely, higher salivary and serum levels of Unc5b than the other groups (P = .002). The GCF levels of Unc5b were significantly reduced (P < .01), and conversely, serum concentrations were significantly increased after NSPT (P < .05). GCF Netrin-1 and Unc5b total amounts were positively correlated with clinical parameters (P < .01 and P < .05) whereas salivary Netrin-1 and Unc5b concentrations were negatively correlated with clinical parameters (P < .01 and P < .05).

CONCLUSIONS:

The results of this study indicate that Netrin-1 and its receptor Unc5b may have essential roles in periodontal inflammation and those can be assumed as useful therapeutic agent to control inflammation and periodontal breakdown.

• Authors: Sadiye Gunpinar, Bilge Meraci, Niyazi Dundar
• Journal: Journal of periodontal research (J Periodontal Res) Vol. 55 Issue 2 Pg. 267-276 (Apr 2020) ISSN: 1600-0765 [Electronic] United States
• PMID: 31769036 (Publication Type: Journal Article)
• Copyright: © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• NTN1 protein, human
• Netrin Receptors
• Receptors, Cell Surface
• UNC5B protein, human
• Netrin-1

Topics

• Case-Control Studies
• Gingival Crevicular Fluid (chemistry)
• Gingivitis
• Humans
• Netrin Receptors (analysis)
• Netrin-1 (analysis)
• Periodontitis (diagnosis)
• Receptors, Cell Surface
• Saliva (chemistry)
• Serum (chemistry)