Gracilin-Derivatives as Lead Compounds for Anti-inflammatory Effects.

Abstract

Gracilins are diterpenes derivative, isolated from the marine sponge Spongionella gracilis. Natural gracilins and synthetic derivatives have shown antioxidant, immunosuppressive, and neuroprotective capacities related to the affinity for cyclophilins. The aim of this work was to study anti-inflammatory and immunosuppressive pathways modulated by gracilin L and two synthetic analogues, compound 1 and 2, on a cellular model of inflammation. In this way, the murine BV2 microglia cell line was used. To carry out the experiments, microglia cells were pre-treated with compounds for 1 h and then stimulated with lipopolysaccharide for 24 h to determine reactive oxygen species production, mitochondrial membrane potential, the release of nitric oxide, interleukin-6 and tumor necrosis factor-α and the expression of Nuclear factor-erythroid 2-related factor 2, Nuclear Factor-κB, the inducible nitric oxide synthase, and the cyclophilin A. Finally, a co-culture of neuron SH-SY5Y and microglia BV2 cells was used to check the neuroprotective effect of these compounds. Cyclosporine A was used as a control of effect. The compounds were able to decrease inflammatory mediators, the expression of inflammatory target proteins as well as they activated anti-oxidative mechanism upon inflammatory conditions. For this reason, natural and synthetic gracilins could be interesting for developing anti-inflammatory drugs.

• Authors: Sandra Gegunde, Amparo Alfonso, Eva Alonso, Rebeca Alvariño, Luis M Botana
• Journal: Cellular and molecular neurobiology (Cell Mol Neurobiol) Vol. 40 Issue 4 Pg. 603-615 (May 2020) ISSN: 1573-6830 [Electronic] United States
• PMID: 31729596 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Diterpenes
• Interleukin-6
• Lipopolysaccharides
• NF-E2-Related Factor 2
• NF-kappa B
• Reactive Oxygen Species
• Tumor Necrosis Factor-alpha
• gracilin L
• Nitric Oxide
• Cyclosporine
• Nitric Oxide Synthase Type II

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, pharmacology)
• Cell Line
• Cell Survival (drug effects)
• Cyclosporine (pharmacology)
• Diterpenes (chemistry, pharmacology)
• Humans
• Interleukin-6 (metabolism)
• Lipopolysaccharides (pharmacology)
• Membrane Potential, Mitochondrial (drug effects)
• Mice
• Microglia (drug effects, metabolism)
• NF-E2-Related Factor 2 (metabolism)
• NF-kappa B (metabolism)
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (metabolism)
• Reactive Oxygen Species (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)