Shengjiang Powder ameliorates myocardial injury in septic rats by downregulating the phosphorylation of P38-MAPK.

Abstract

Sepsis is the systemic inflammatory response caused by infection. Cardiac dysfunction is an acknowledged result of sepsis. Shengjiang Powder, a prescribed traditional Chinese medicine, showed anti-infection and antipyretic functions in our previous study. In this study, we established a septic rat model via cecal ligation puncture (CLP) to evaluate the effects of Shengjiang Powder on sepsis and the involvement of P38 mitogen activated protein kinase (P38-MAPK) signaling. The 10 main ingredients of Shengjiang Powder were identified by LC-MS. The results of this study indicated that Shengjiang Powder at a concentration of 3.0 g/kg with SB203580 (an inhibitor of P38-MAPK) could improve myocardial injury, ameliorate the histopathological abnormalities, decrease apoptosis and upregulate proliferating cell nuclear antigen (PCNA) levels in myocardial tissues. Further, cytokine mRNA expression levels (tumor necrosis factor - alpha, TNF-α and interleukin 6, IL-6) were decreased by Shengjiang Powder and SB203580 in the myocardial tissues. Furthermore, the p-P38 protein level in myocardial tissues was upregulated in septic rats but decreased upon treatment with Shengjiang Powder and SB203580; however, the relative protein level of P38 showed no significant changes. Collectively, Shengjiang Powder showed a myocardial protective effect on rats with CLP-induced sepsis.

• Authors: Yiming Qian, Fenghua Qian, Weiwei Zhang, Lei Zhao, Mengwen Shen, Chunlei Ding, Jian Guo
• Journal: Journal of biosciences (J Biosci) Vol. 44 Issue 2 (Jun 2019) ISSN: 0973-7138 [Electronic] India
• PMID: 31180053 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Drugs, Chinese Herbal
• Il6 protein, rat
• Imidazoles
• Interleukin-6
• NF-kappa B
• Proliferating Cell Nuclear Antigen
• Pyridines
• Tumor Necrosis Factor-alpha
• p38 Mitogen-Activated Protein Kinases
• SB 203580

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, pharmacology)
• Apoptosis (drug effects, genetics)
• Disease Models, Animal
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Gene Expression Regulation
• Heart Injuries (genetics, metabolism, pathology, prevention & control)
• Imidazoles (pharmacology)
• Interleukin-6 (genetics, metabolism)
• Male
• Myocardium (metabolism, pathology)
• Myocytes, Cardiac (drug effects, metabolism, pathology)
• NF-kappa B (genetics, metabolism)
• Phosphorylation (drug effects)
• Proliferating Cell Nuclear Antigen (genetics, metabolism)
• Pyridines (pharmacology)
• Rats
• Rats, Wistar
• Sepsis
• Signal Transduction
• Tumor Necrosis Factor-alpha (genetics, metabolism)
• p38 Mitogen-Activated Protein Kinases (antagonists & inhibitors, genetics, metabolism)