Protective effect of a polyphenols-rich extract from Inonotus Sanghuang on bleomycin-induced acute lung injury in mice.

Abstract

Mushroom Phellinus linteus ("Sanghuang" in Chinese) is a popular medicinal polypore used to treat several disorders through its various biological functions. Inonotus sanghuang is claimed to produce general immune-potentiating and strengthening, anti-inflammatory, anti-tumor and anti-microbial properties, but its effect on acute lung inflammation and oxidative stress are not clearly understood. To determine the effect and mechanism of the polyphenols-rich ethyl acetate fraction from wild I. sanghuang extract (ISE) on acute lung injury (ALI) induced by bleomycin (BLM), female C57BL/6 mice were fed ISE (0%, 0.15% or 0.6% in diet) for 4 weeks prior to challenge with BLM. Bronchoalveolar lavage fluid (BALF) from lung, spleen and lung tissues were collected on day 3 after BLM challenge for histological, oxidative stress, molecular and biochemical analysis. ISE supplementation improved pathological features in lung injury scores and reduced lung wet-to-dry ratios. Moreover, ISE reduced inflammatory cell infiltration and the pro-inflammatory cytokines including IL-1β, IL-6 and TNF-α in BALF, decreased the MPO activity and the MDA level and increased the SOD, CAT and GSH-Px activities in lung tissue homogenates. Further mechanism analysis demonstrated that dietary ISE inhibited NF-κB signal. Finally, peripheral immune function analysis showed that ISE had less effect on immune response including splenocyte producing inflammatory cytokines and T cell proliferation except for IL-1β and IL-2. Our findings indicate the possibility that dietary ISE attenuates ALI induced by BLM through correcting the inflammation and oxidation balance at least in part via inhibiting NF-κB signal in vivo, suggesting that ISE might be a valuable medicinal food effective in improving lung injury.

• Authors: Xing Su, Kun Liu, Yu Xie, Mengdi Zhang, Yong Wang, Min Zhao, Yilin Guo, Yijie Zhang, Junpeng Wang
• Journal: Life sciences (Life Sci) Vol. 230 Pg. 208-217 (Aug 01 2019) ISSN: 1879-0631 [Electronic] Netherlands
• PMID: 31152815 (Publication Type: Journal Article)
• Copyright: Copyright © 2019. Published by Elsevier Inc.

Chemical References

• Antioxidants
• Cytokines
• Interleukin-1beta
• NF-E2-Related Factor 2
• NF-kappa B
• Phellinus linteus extract
• Plant Extracts
• Polyphenols
• Tumor Necrosis Factor-alpha
• Bleomycin

Topics

• Acute Lung Injury (chemically induced, drug therapy)
• Agaricales (isolation & purification, metabolism)
• Animals
• Antioxidants (pharmacology)
• Bleomycin (pharmacology)
• Bronchoalveolar Lavage Fluid (cytology)
• Cytokines (metabolism)
• Female
• Inflammation (pathology)
• Interleukin-1beta (pharmacology)
• Lung (pathology)
• Mice
• Mice, Inbred C57BL
• NF-E2-Related Factor 2 (metabolism)
• NF-kappa B (metabolism)
• Oxidative Stress (drug effects)
• Phellinus
• Plant Extracts (pharmacology)
• Polyphenols (isolation & purification, pharmacology)
• Signal Transduction (drug effects)
• Tumor Necrosis Factor-alpha (pharmacology)