Cardioprotective effects of alternagin-C (ALT-C), a disintegrin-like protein from Rhinocerophis alternatus snake venom, on hypoxia-reoxygenation-induced injury in fish.

Abstract

Alternagin-C (ALT-C) is a disintegrin-like peptide purified from Rhinocerophis alternatus snake venom with the property of inducing vascular endothelial growth factor (VEGF) expression, endothelial cell proliferation and migration, and angiogenesis. Therefore, this protein could be interesting as a new approach for ischemic heart diseases, an imbalance between myocardial oxygen supply and demand, leading to cardiac dysfunction. We investigated the effects of a single dose of alternagin-C (0.5 mg kg-1, via intra-arterial), after 7 days, on hypoxia/reoxygenation challenge in isolated ventricle strips and on morphological changes and density of blood vessels of the heart, using fish as an alternative experimental model. ALT-C treatment provided protection of cardiomyocytes against hypoxia/reoxygenation-induced negative inotropism. ALT-C also stimulated angiogenesis and improved excitation-contraction coupling during hypoxic conditions. Our results provide a new insight into a functional role of ALT-C against hypoxia/reoxygenation-induced cardiomyocyte injury pointing out to a potential therapeutic strategy for ischemia-related diseases.

• Authors: D A Monteiro, A L Kalinin, H S Selistre-de-Araújo, L A N Nogueira, M E Beletti, M N Fernandes, F T Rantin
• Journal: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP (Comp Biochem Physiol C Toxicol Pharmacol) Vol. 215 Pg. 67-75 (Jan 2019) ISSN: 1532-0456 [Print] United States
• PMID: 30352307 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Inc. All rights reserved.

Chemical References

• Angiogenesis Inducing Agents
• Cardiotonic Agents
• Crotalid Venoms
• Disintegrins
• Fish Proteins
• Reptilian Proteins
• alternagin-C
• Collagen

Topics

• Angiogenesis Inducing Agents (administration & dosage, therapeutic use)
• Animals
• Aquaculture
• Bothrops
• Cardiotonic Agents (administration & dosage, therapeutic use)
• Characiformes
• Collagen (metabolism)
• Coronary Vessels (drug effects, pathology, physiopathology)
• Crotalid Venoms (chemistry)
• Disease Models, Animal
• Disintegrins (administration & dosage, therapeutic use)
• Excitation Contraction Coupling (drug effects)
• Fish Proteins (metabolism)
• Heart (drug effects, physiology, physiopathology)
• Injections, Intra-Arterial
• Myocardial Contraction (drug effects)
• Myocardial Reperfusion Injury (pathology, physiopathology, prevention & control)
• Myocardium (metabolism, pathology)
• Neovascularization, Physiologic (drug effects)
• Reptilian Proteins (administration & dosage, therapeutic use)