The effects of S-nitrosoglutathione on intestinal ischemia reperfusion injury and acute lung injury in rats: Roles of oxidative stress and NF-κB.

Abstract

BACKGROUND:

Intestinal ischemia and reperfusion (I/R) induces oxidative stress, inflammatory response, and acute lung injury. S-nitrosoglutathione (GSNO), a nitric oxide donor, has been documented to have protective effects on experimental ischemia models.

AIM:

The aim of this study was to examine the effect of GSNO on I/R-induced intestine and lung damage and detect the potential mechanisms emphasizing the protective role of GSNO.

METHODS:

Intestinal I/R was induced by occluding the superior mesenteric artery for 30 min followed by reperfusion for 180 min. GSNO was administered intravenously before reperfusion period (0.25 mg/kg). The levels of lipid peroxidation, reduced glutathione, and myeloperoxidase (MPO), histopathological evaluation and immunohistochemical expressions of both nuclear factor KappaB (NF-κB) and inducible nitric oxide (iNOS) in intestine and lung tissues were assessed.

RESULTS:

Histolopathologic evaluation demonstrated that intestinal I/R induced severe damages in the intestine and the lung tissues. Histopathological scores decreased with GSNO treatment. GSNO treatment reduced lipid peroxidation and MPO levels and inhibited expression of NF-κB and iNOS in the intestine.

CONCLUSION:

Our results suggest that GSNO treatment may ameliorate the intestinal and lung injury in rats, at least in part, by inhibiting inflammatory response and oxidative stress.

• Authors: Inci Turan, Hale Sayan Ozacmak, V Haktan Ozacmak, Figen Barut, I Diler Ozacmak
• Journal: Tissue & cell (Tissue Cell) Vol. 52 Pg. 35-41 (Jun 2018) ISSN: 1532-3072 [Electronic] Scotland
• PMID: 29857826 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Ltd. All rights reserved.

Chemical References

• NF-kappa B
• Nitric Oxide Donors
• S-Nitrosoglutathione

Topics

• Acute Lung Injury (etiology, metabolism)
• Animals
• Intestines (drug effects, pathology)
• Male
• NF-kappa B (metabolism)
• Nitric Oxide Donors (pharmacology)
• Oxidative Stress (drug effects)
• Rats
• Rats, Wistar
• Reperfusion Injury (complications, metabolism)
• S-Nitrosoglutathione (pharmacology)