Changes in circulating cell-free DNA and nucleosomes in patients with exacerbated psoriasis.

Abstract

Psoriasis is a multifactorial chronic inflammatory disease. We aimed to examine blood levels of nucleosomes derived from apoptotic cells, nucleosomal cell-free DNA (cfDNA) and immune-inflammatory biomarkers tumor necrosis factor-α (TNF-α), C-reactive protein (CRP), and interleukin 6 (IL-6) in psoriatic subjects. The study included 28 patients with exacerbated psoriasis vulgaris and 22 controls. The clinical and laboratory investigations included the determination of PASI score, BMI, cfDNA (by real-time PCR), nucleosomes, TNF-α, CRP, and IL-6. The range of PASI score in psoriatic patients was 10-34 (median 19). In the patients, we found significantly elevated levels (p < 0.001) of cfDNA, nucleosomes, TNF-α, CRP, and IL-6. We did not find any significant relationship between the analyzed parameters in either group (i.e., experimental or control). Elevated levels of the biomarkers of inflammation (TNF-α, CRP, and IL-6) and the indicators of apoptosis (cfDNA, circulating nucleosomes) proved that exacerbated psoriasis vulgaris is associated with a high degree of systemic inflammatory responses and dysregulated apoptotic pathways.

• Authors: Martin Beranek, Zdenek Fiala, Jan Kremlacek, Ctirad Andrys, Jan Krejsek, Kvetoslava Hamakova, Marcela Chmelarova, Vladimir Palicka, Lenka Borska
• Journal: Archives of dermatological research (Arch Dermatol Res) Vol. 309 Issue 10 Pg. 815-821 (Dec 2017) ISSN: 1432-069X [Electronic] Germany
• PMID: 29027583 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• Cell-Free Nucleic Acids
• IL6 protein, human
• Interleukin-6
• Nucleosomes
• Tumor Necrosis Factor-alpha
• C-Reactive Protein

Topics

• Adult
• Aged
• Apoptosis
• Biomarkers (blood)
• C-Reactive Protein (analysis)
• Cell-Free Nucleic Acids (blood)
• Disease Progression
• Female
• Humans
• Inflammation
• Interleukin-6 (blood)
• Male
• Middle Aged
• Nucleosomes (genetics, metabolism)
• Psoriasis (blood, pathology)
• Severity of Illness Index
• Tumor Necrosis Factor-alpha (blood)
• Young Adult