Increased
sugar consumption has been proposed to be a risk factor for
obesity-related metabolic disorders. The objective of this study was to investigate the anti-inflammatory effect of
turanose in Raw 264.7 macrophages.
Turanose (3-O-α-D-glucosyl-D-fructose), an isomer of
sucrose, naturally exists in honey. For these studies, macrophages were treated with total
glucose (Glu), 50% Glu/50%
turanose (T50), 25% Glu/75%
turanose (T75), and 100%
turanose (T100), each with a total concentration of 25 mM in cell media. Expressions of inflammatory
enzymes and
cytokines were analyzed. Cell viability was not affected in the
turanose treated groups compared to the Glu group.
Lipopolysaccharide and
glucose-induced
nitric oxide production,
protein expression of
inducible nitric oxide synthase, COX-2, and
superoxide dismutase 2, and
mRNA expression levels of
interleukin (IL)-1β and
IL-18 were significantly suppressed by
turanose treatment. These results demonstrate that
turanose exerts anti-inflammatory effects in vitro, and possesses potential to serve therapeutic functional
sweetener for testing in vivo and in clinical trials.