Abstract | BACKGROUND: Currently available diagnostic techniques of Plasmodium falciparum infection are not optimal for non-invasive, population-based screening for malaria. It was hypothesized that a mass spectrometry-based metabolomics approach could identify urinary biomarkers of falciparum malaria. METHODS: The study used a case-control design, with cases consisting of 21 adults in central Ethiopia with a diagnosis of P. falciparum infection confirmed with microscopy, and 25 controls of adults with negative blood smears for malaria matched on age and sex. Urinary samples were collected from these individuals during presentation at the clinic, and a second sample was collected from both cases and controls 4 weeks later, after the cases had received anti-malarial medication. The urine samples were screened for small molecule urinary biomarkers, using mass spectrometry-based metabolomics analyses followed by multivariate analysis using principal component analysis and orthogonal partial least square-discriminant analysis. The chemical identity of statistically significant malaria biomarkers was confirmed using tandem mass spectrometry. RESULTS: CONCLUSION: The urinary biomarkers of malaria identified have potential for the development of non-invasive and rapid diagnostic test of P. falciparum infection.
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Authors | Salah Abdelrazig, Catharine A Ortori, Gail Davey, Wakgari Deressa, Dhaba Mulleta, David A Barrett, Alemayehu Amberbir, Andrew W Fogarty |
Journal | Malaria journal
(Malar J)
Vol. 16
Issue 1
Pg. 229
(05 30 2017)
ISSN: 1475-2875 [Electronic] England |
PMID | 28558710
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adolescent
- Adult
- Antimalarials
(therapeutic use)
- Biomarkers
(urine)
- Case-Control Studies
- Ethiopia
- Female
- Humans
- Malaria, Falciparum
(diagnosis, metabolism, parasitology)
- Male
- Mass Spectrometry
- Metabolome
- Metabolomics
(methods)
- Middle Aged
- Multivariate Analysis
- Plasmodium falciparum
(isolation & purification)
- Young Adult
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