Establishment and characterization of a squamous cell carcinoma cell line, designated hZK-1, derived from a metastatic lymph node tumor of the tongue.

Abstract

The hZK-1 cell line was successfully established from the metastatic foci of a lymph node of an 82-year-old Japanese woman with squamous cell carcinoma of the tongue. The pathological diagnosis of the tumor was moderately to well-differentiated squamous cell carcinoma. The hZK-1 cells were angular in shape, and had neoplastic and pleomorphic features. Adjacent hZK-1 cells were joined by desmosomes and well-developed microvilli, and many free ribosomes were observed in the cytoplasm. The doubling time of the hZK-1 cells was approximately 36, 33, and 29 h at the 10th, 20th, and 30th passages, respectively. The cell line was shown to be triploid, with a chromosomal distribution of 75-80. Immunocytochemical staining of the hZK-1 cells revealed cytokeratin (CK) 17-, Ki67-, and p53-positive staining, and negative staining for CK13. The hZK-1 cells were negative for human papillomavirus (HPV)-16 or-18 infection. Grafting was not successful when the hZK-1 cells were transplanted into the subcutis of SCID mice. The hZK-1 cells (2 × 106 cells/3 ml of growth medium) secreted vascular endothelial growth factor (VEGF) that reached a concentration of 2.6 ng/ml media after 3 days of culture. Hypoxia enhanced cellular HIF-1α expression and VEGF secretion in hZK-1 cells. The HIF-1α inhibitor YC-1 partially inhibited hypoxia-induced VEGF secretion in ZK-1 cells. The reverse transcription-polymerase chain reaction (RT-PCR) results revealed that the expression of CK17, Ki67, and p53 was elevated in the hZK-1 cells. hZK-1 cells were not sensitive to CDDP, TXT, 5-FU, or a mixture of these three anti-tumor agents.

• Authors: Tetsuya Hirabayashi, Haruka Takahashi, Miho Watanabe, Toshiaki Tachibana
• Journal: Human cell (Hum Cell) Vol. 30 Issue 4 Pg. 319-326 (Oct 2017) ISSN: 1749-0774 [Electronic] Japan
• PMID: 28260147 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• HIF1A protein, human
• Hypoxia-Inducible Factor 1, alpha Subunit
• Indazoles
• Keratin-13
• Keratin-17
• Ki-67 Antigen
• Tumor Suppressor Protein p53
• Vascular Endothelial Growth Factor A
• 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole

Topics

• Aged, 80 and over
• Animals
• Antineoplastic Agents (pharmacology)
• Carcinoma, Squamous Cell (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Drug Resistance, Neoplasm
• Female
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (antagonists & inhibitors, metabolism)
• Indazoles (pharmacology)
• Keratin-13 (metabolism)
• Keratin-17 (metabolism)
• Ki-67 Antigen (metabolism)
• Lymphatic Metastasis
• Mice, SCID
• Tongue Neoplasms (genetics, metabolism, pathology)
• Tumor Suppressor Protein p53 (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)