De novo expression in the kidney of
periostin, a
protein involved in odontogenesis and osteogenesis, has been suggested as a
biomarker of renal disease. In this study, we investigated the mechanism(s) of induction and the role of
periostin in renal disease. Using a combination of bioinformatics, reporter assay, and
chromatin immunoprecipitation analyses, we found that NFκB and other proinflammatory
transcription factors induce
periostin expression in vitro and that binding of these factors on the
periostin promoter is enriched in glomeruli during experimental GN. Mice lacking expression of
periostin displayed preserved renal function and structure during GN. Furthermore, delayed administration of
periostin antisense oligonucleotides in wild-type animals with GN reversed already established
proteinuria, diminished tissue
inflammation, and improved renal structure. Lack of
periostin expression also blunted the de novo renal expression of integrin-β3 and phosphorylation of
focal adhesion kinase and AKT, known mediators of integrin-β3 signaling that affect cell motility and survival, observed during GN in wild-type animals. In vitro, recombinant
periostin increased the expression of integrin-β3 and the concomitant phosphorylation of
focal adhesion kinase and AKT in podocytes. Notably,
periostin and integrin-β3 were highly colocalized in biopsy specimens from patients with inflammatory GN. These results demonstrate that interplay between
periostin and renal
inflammation orchestrates inflammatory and fibrotic responses, driving podocyte damage through downstream activation of integrin-β3 signaling. Targeting
periostin may be a novel therapeutic strategy for treating CKD.