Protein Inhibitor of Activated STAT3 Suppresses Oxidized LDL-induced Cell Responses during Atherosclerosis in Apolipoprotein E-deficient Mice.

Abstract

Atherosclerosis is a serious public health concern. Excessive inflammatory responses of vascular cells are considered a pivotal pathogenesis mechanism underlying atherosclerosis development. It is known that Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) signalling plays an important role in atherosclerosis progression. Protein inhibitor of activated STAT3 (PIAS3) is the key negative regulator of JAK/STAT3 signalling. However, its effect on atherogenesis is unknown. Here, we observed that PIAS3 levels are reduced in atherosclerotic lesions and that PIAS3 expression decreases in conjunction with increases in interleukin-6 expression and atherosclerosis severity. Oxidized low-density lipoprotein (ox-LDL), an atherogenic stimulus, reduced PIAS3 expression, an effect that may be attributed to nitric oxide synthesis upregulation. In turn, PIAS3 overexpression effectively suppressed ox-LDL-induced inflammation, lipid accumulation and vascular smooth muscle cell proliferation. These results indicate that PIAS3 is a critical repressor of atherosclerosis progression. The findings of this study have contributed to our understanding on the pathogenesis of atherosclerosis and have provided us with a potential target through which we can inhibit atherosclerosis-related cellular responses.

• Authors: Rong Wang, Yanjin Zhang, Liran Xu, Yan Lin, Xiaofeng Yang, Liang Bai, Yulong Chen, Sihai Zhao, Jianglin Fan, Xianwu Cheng, Enqi Liu
• Journal: Scientific reports (Sci Rep) Vol. 6 Pg. 36790 (11 15 2016) ISSN: 2045-2322 [Electronic] England
• PMID: 27845432 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoproteins E
• Interleukin-6
• Lipoproteins, LDL
• Pias3 protein, mouse
• Protein Inhibitors of Activated STAT
• interleukin-6, mouse
• oxidized low density lipoprotein
• Nitric Oxide

Topics

• Animals
• Apolipoproteins E (deficiency)
• Atherosclerosis (genetics, immunology, metabolism)
• Cell Proliferation
• Disease Models, Animal
• Down-Regulation
• Interleukin-6 (metabolism)
• Lipoproteins, LDL (metabolism)
• Mice
• Muscle, Smooth, Vascular (cytology, metabolism)
• Myocytes, Smooth Muscle (cytology, metabolism)
• Nitric Oxide (metabolism)
• Protein Inhibitors of Activated STAT (metabolism)
• RAW 264.7 Cells
• Signal Transduction