Pim-3 is a Critical Risk Factor in Development and Prognosis of Prostate Cancer.

Abstract

BACKGROUND Pim-3 kinase is a highly homologous serine/threonine kinase that is overexpressed in hematological malignancies and solid tumors. Few studies have been conducted to define the role of Pim-3 in solid tumors, especially in prostate cancer. The aim of this study was to define the role of Pim-3 in development and prognosis of prostate cancer. MATERIAL AND METHODS We collected specimens from 160 patients with prostate cancer, as well as 100 patients with benign prostatic hyperplasia. Realtime polymerase chain reaction was used for the assessment of Pim-3 expression at the RNA level and Western blot was used to quantify the Pim-3 protein synthesis in 3 different cell lines. RESULTS We found that Pim-3 mRNA expression in prostate cancer tissue was significantly higher than that in benign prostatic hyperplasia tissue (p<0.05). Accordingly, the protein level expression of Pim-3 in prostate cancer cell lines was also significantly higher than that in control cells. In addition, the expression status of Pim-3 mRNA was significantly associated with pathological parameters such as pre-surgery prostate specific antigen, Gleason score, pathological stage, and lymphoid metastasis. High expression of Pim-3 also significantly decreased the survival rate of patients after surgery. CONCLUSIONS Pim-3 expression is an important risk factor for prostate cancer; we are the first team to report Pim-3 as a valuable biomarker in Chinese.

• Authors: Yanchun Qu, Changwen Zhang, E Du, Andi Wang, Yuming Yang, Jianing Guo, Aixiang Wang, Zhihong Zhang, Yong Xu
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 22 Pg. 4254-4260 (Nov 09 2016) ISSN: 1643-3750 [Electronic] United States
• PMID: 27826135 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Proto-Oncogene Proteins
• RNA, Messenger
• PIM3 protein, human
• Protein Serine-Threonine Kinases

Topics

• Aged
• Aged, 80 and over
• Biomarkers, Tumor (blood, genetics, metabolism)
• Case-Control Studies
• Cell Line, Tumor
• China
• Humans
• Male
• Middle Aged
• Prognosis
• Prostatic Hyperplasia (enzymology, genetics, metabolism)
• Prostatic Neoplasms (enzymology, genetics, metabolism)
• Protein Serine-Threonine Kinases (biosynthesis, blood, genetics, metabolism)
• Proto-Oncogene Proteins (biosynthesis, blood, genetics, metabolism)
• RNA, Messenger (biosynthesis, genetics, metabolism)
• Risk Factors